W/O/W型复乳的制备优化及包埋矢车菊素-3-葡萄糖苷效果分析

研究制备方法和乳化剂对W/O/W型复乳稳定性的影响，优化工艺参数，并研究体系包埋矢车菊素-3-葡萄糖苷（cyanidin-3-glucoside，C-3-G）的效果。得到优化条件：初乳搅拌速率15 500 r/min、复乳搅拌速率7 000 r/min、油相乳化剂聚甘油蓖麻醇酯添加量16%、亲水性乳化剂的亲水亲油平衡值10、亲水性乳化剂添加量10%，优化后离心保留率达到80.25%。利用优化得到的复乳体系包裹C-3-G，发现复乳对C-3-G有良好的包裹能力，复乳对C-3-G的载药量为1 500 mg/L，包封率为98.08%，且C-3-G的包埋提高内水相渗透压和复乳稳定性。

Optimization of Preparation of W/O/W Multiple Emulsion and Evaluation of Its Ability to Encapsulate Cyanidin-3-Glucoside

In this experiment, the effects of stirring rate and emulsifier type on the stability of W/O/W double emulsion were studied in order to optimize the process parameters. The ability of W/O/W double emulsion to encapsulate cyanidin-3- glucoside (C-3-G) was evaluated as well. The optimum conditions were found to be as follows: stirring rate 15 500 r/min for the first emulsification, 7 000 r/min for the second emulsification, polyglycerol ricinoleate concentration (PGPR, oil phase emulsifier) 16%, hydrophile lipophile balance (HLB) of hydrophilic emulsifier 10, and hydrophilic emulsifier concentration 10%. Under these conditions, the retention rate after centrifugation reached 80.25%. C-3-G was well encapsulated by the optimized multiple emulsion. It was found that the drug-loading rate of the emulsion was 1 500 mg/L and the encapsulation efficiency was 98.08%. The encapsulation of C-3-G improved the emulsion stability by increasing the viscosity of the inner water phase.