槟榔籽多酚对肠道微生物体外发酵的影响

通过间歇式静态厌氧体外发酵技术研究鲜槟榔籽中多酚类物质对青年健康男性肠道微生物的影响。采集24岁健康男性的粪便为菌源,分别以对照组(C组)、添加了纯度为74.09%的槟榔籽多酚的试验组(P组)为底物分别进行2,6,12,24h4个时间段的体外发酵。利用气相色谱、高通量测序等方法对pH值、多酚成分、短链脂肪酸(SCFAs)和微生物指标进行测定。结果显示,槟榔籽多酚对青年健康男性肠道微环境中pH变化趋势影响不大,但会降低SCFAs的产量。肠道菌群方面,可以提高其肠道菌群的物种多样性水平,改变变形菌门(Proteobacteria)与厚壁菌门(Firmicutes)相对丰度比值,其中柔嫩梭菌属(Faecalibacterium)增加幅度最大,拟杆菌属(Bacteroides)和双歧杆菌属(Bifidobacterium)的相对丰度也有小幅增加。研究表明槟榔籽多酚能改变肠道菌群结构,但暂未发现与其他植物多酚类似的功效。

Effects of polyphenols from Areca seed on intestinal microbes in vitro

This study aimed to investigating the effects of the polyphenols from Areca seeds on young men’s intestinal microbes in vitro through intermittent static anaerobic fermentation technology.The feces of 24 years healthy young men are taken as starter.The substrates of the control group(group C)and the experimental group(group P)with a purity of 74.09% were fermented in vitro at 2,6,12 and 24 h.pH value,polyphenol composition,short chain fatty acid(SCFAs)and microorganism index were measured by gas chromatography and highthroughput sequencing.The results showed that the polyphenols of the Areca-nut polyphenols had little effect on the change of pH in the intestinal microenvironment of young healthy men,but it reduced the content of SCFAs.In terms of intestinal flora,it could improve the species diversity level of its intestinal flora,reduce the relative abundance of Proteobacteria,and improve the relative abundance of Firmicutes.Among them,the largest increase is Faecalibacterium, while the relative abundance of Bacteroides and Bifidobacterium was also slightly increased.The above results showed that betel nut polyphenols could change the structure of intestinal flora,but no similar health effects was found.