| 基于固相基质分散的高效液相色谱-串联质谱法测定茄科蔬菜及其制品中龙葵素的含量 |
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| 引用本文： | 唐丽君,匡佩琳,李雨露,张文中,曾林晖,朱作为.基于固相基质分散的高效液相色谱-串联质谱法测定茄科蔬菜及其制品中龙葵素的含量[J].食品安全质量检测技术,2019,10(17):5827-5836. |
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| 作者姓名： | 唐丽君 匡佩琳 李雨露 张文中 曾林晖 朱作为 |
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| 作者单位： | 江西省食品检验检测研究院 |
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| 基金项目： | 江西省食品药品监督管理局科技项目(2016SP12) |
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| 摘 要： | 目的建立高效液相色谱-串联质谱法测定茄科蔬菜及其制品中龙葵素α-茄碱和α-卡茄碱含量。方法样品采用0.1%甲酸水溶液:乙腈=1:1(V:V)提取,C18固相基质分散净化,经过色谱柱BEHAmide (2.1mm×100 mm, 1.7μm)分离,以2 mmol/L乙酸铵的0.1%甲酸水溶液:2 mmol/L乙酸铵的乙腈溶液为流动相进行梯度洗脱,柱温40℃,流速0.4m L/min,多反应模式监测,外标法定量。结果α-茄碱和α-卡茄碱在5～1000ng/m L内线性关系良好(r20.999)。α-茄碱方法检出限和定量限范围分别为0.1～1 mg/kg, 0.3～3 mg/kg;α-卡茄碱检出限和定量限分别为0.03～0.3 mg/kg, 0.1～1 mg/kg;在低、中、高3水平添加下,α-茄碱回收率为90.7%～108.0%,α-卡茄碱回收率为89.8%～102.7%,相对标准偏差均小于5.6%(n=6)。结论本方法快速简便、准确度好、灵敏度高等特点,适用于大批量茄科蔬菜及其制品中α-茄碱和α-卡茄碱的测定。
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| 关 键 词： | 茄科蔬菜 龙葵素 固相基质分散 高效液相色谱-串联质谱法 |
| 收稿时间： | 2019/6/26 0:00:00 |
| 修稿时间： | 2019/9/16 0:00:00 |
Determination of solanine in solanaceous vegetables and products of solanaceous vegetable by high performance liquid chromatography-tandem mass spectrometry based on solid phase matrix dispersion |
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| TANG Li-Jun,KUANG Pei-Lin,LI Yu-Lu,ZHANG Wen-Zhong,ZENG Lin-Hui and ZHU Zuo-Wei.Determination of solanine in solanaceous vegetables and products of solanaceous vegetable by high performance liquid chromatography-tandem mass spectrometry based on solid phase matrix dispersion[J].Food Safety and Quality Detection Technology,2019,10(17):5827-5836. |
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| Authors: | TANG Li-Jun KUANG Pei-Lin LI Yu-Lu ZHANG Wen-Zhong ZENG Lin-Hui and ZHU Zuo-Wei |
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| Affiliation: | Food Inspection and Testing Institute of Jiangxi Province,Food Inspection and Testing Institute of Jiangxi Province,Food Inspection and Testing Institute of Jiangxi Province,Food Inspection and Testing Institute of Jiangxi Province,Food Inspection and Testing Institute of Jiangxi Province and Food Inspection and Testing Institute of Jiangxi Province |
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| Abstract: | |
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| Keywords: | solanaceous vegetables solanine matrix solid-phase disperse high performance liquid chromatography- tandem mass spectrometry |
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