| 高效液相色谱-串联质谱法快速检测银耳中的米酵菌酸 |
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| 引用本文： | 曾令浩,李思颖,李文倩,李柏衡,岑建斌,区硕俊. 高效液相色谱-串联质谱法快速检测银耳中的米酵菌酸[J]. 食品安全质量检测学报, 2019, 10(19): 6538-6541 |
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| 作者姓名： | 曾令浩 李思颖 李文倩 李柏衡 岑建斌 区硕俊 |
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| 作者单位： | 广州检验检测认证集团有限公司国家加工食品质量检验中心(广东);广州质量监督检测研究院 |
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| 摘 要： | 目的建立高效液相色谱-串联质谱法(highperformanceliquidchromatography-tandemmass spectrometry,HPLC-MS/MS)快速检测银耳中的米酵菌酸的方法。方法样品以甲醇提取后,超声振荡,离心分层,上清液用甲醇定容。使用空白基质液配置标准曲线对结果进行校正。在优化后的色谱及质谱条件下,采用(electronsprayionization,ESI)负离子模式进行电离,并通过多反应监测(multi-selectedreactionmonitoring,MRM)模式对目标化合物的定量离子和定性离子进行测定。结果米酵菌酸在0.50～50.00μg/L范围内线性良好,相关系数r为0.9996,3个浓度添加水平的平均回收率范围在80.6%～95.6%之间,相对标准偏差为4.98%～7.21%。方法检出限(limit of detection, LOD)为5.0μg/kg,定量限(limit of quantification, LOQ)为15.0μg/kg。结论该方法操作简便快速,回收率高、灵敏度高、精密度好,适合测定银耳基质中的米酵菌酸。
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| 关 键 词： | 高效液相色谱-串联质谱 银耳 米酵菌酸 外标法 |
| 收稿时间： | 2019-05-27 |
| 修稿时间： | 2019-07-13 |
Rapid determination of bongkrekic acid in Tremella by high performance liquid chromatography-tandem mass spectrometry |
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| ZENG Ling-Hao,LI Si-Ying,LI Wen-Qian,LI Bo-Heng,CEN Jian-Bin and OU Shuo-Jun. Rapid determination of bongkrekic acid in Tremella by high performance liquid chromatography-tandem mass spectrometry[J]. Journal of Food Safety & Quality, 2019, 10(19): 6538-6541 |
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| Authors: | ZENG Ling-Hao LI Si-Ying LI Wen-Qian LI Bo-Heng CEN Jian-Bin OU Shuo-Jun |
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| Affiliation: | Guangzhou Inspection and Testing Certification Group Co., National Quality Testing Center for Processed Food (Guangdong),Guangzhou Inspection and Testing Certification Group Co., National Quality Testing Center for Processed Food (Guangdong),Guangzhou Inspection and Testing Certification Group Co., National Quality Testing Center for Processed Food (Guangdong),Guangzhou Quality Supervision and Testing Institute,Guangzhou Inspection and Testing Certification Group Co., National Quality Testing Center for Processed Food (Guangdong) and Guangzhou Inspection and Testing Certification Group Co., National Quality Testing Center for Processed Food (Guangdong) |
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| Abstract: | |
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| Keywords: | high performance liquid chromatography-tandem mass spectrometry Tremella bongkrekic acid external standard method |
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