| 微波辅助快速制备碳量子点及其对表没食子儿茶素-3-没食子酸酯的促氧化作用 |
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| 引用本文： | 吴春莲,蒲洪彬,曲佳欢,孙大文,韦庆益.微波辅助快速制备碳量子点及其对表没食子儿茶素-3-没食子酸酯的促氧化作用[J].食品科学,2019,40(5):63-69. |
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| 作者姓名： | 吴春莲 蒲洪彬 曲佳欢 孙大文 韦庆益 |
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| 作者单位： | 华南理工大学食品科学与工程学院，现代食品工程研究中心，广东省冷链食品智能感知与过程控制工程技术研究中心，广东 广州 510006 |
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| 基金项目： | 中央高校基本科研业务费专项资金项目（2017MS075）；广东省农业厅农产品保鲜物流共性技术创新团队项目（2016LM2154）；广州市协同创新重大专项（201604020007；201604020057） |
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| 摘 要： | 利用高通量微波消解/萃取工作站快速制备水溶性碳量子点，研究碳量子点的pH值依赖性和稳定性，探讨表没食子儿茶素-3-没食子酸酯（epigallocatechin-3-gallate，EGCG）在Cu2＋存在条件下产生的活性氧自由基对碳量子点荧光探针的猝灭作用及EGCG与Cu2＋的促氧化剂量-效应关系。结果表明：碳量子点具有pH值依赖性和良好的稳定性，相对量子产率为73.67%；Cu2＋与EGCG的浓度比达到2∶1时，碳量子点荧光探针的猝灭更严重，EGCG的促氧化效果更好。该研究可为EGCG作为功能性物质的应用提供理论依据。
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| 关 键 词： | 碳量子点荧光探针 表没食子儿茶素-3-没食子酸酯 促氧化作用 剂量-效应关系 |
Rapid Microwave-Assisted Preparation of Carbon Quantum Dots and Its Application for Investigating Pro-oxidative Activity of Epigallocatechin-3-gallate |
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| WU Chunlian,PU Hongbin,QU Jiahuan,SUN Da-wen,WEI Qingyi.Rapid Microwave-Assisted Preparation of Carbon Quantum Dots and Its Application for Investigating Pro-oxidative Activity of Epigallocatechin-3-gallate[J].Food Science,2019,40(5):63-69. |
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| Authors: | WU Chunlian PU Hongbin QU Jiahuan SUN Da-wen WEI Qingyi |
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| Affiliation: | Academy of Contemporary Food Engineering, Engineering and Technological Research Centre of Guangdong Province on Intelligent Sensing and Process Control of Cold Chain Foods, School of Food Science and Engineering, South China University of Technology, Guangzhou 510006, China |
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| Abstract: | Aqueous carbon quantum dots were prepared rapidly by high-throughput microwave digestion/extraction workstation, and their pH dependence and stability behavior were studied. The fluorescence quenching of carbon quantum dots by reactive oxygen species produced by epigallocatechin-3-gallate (EGCG) in the presence of Cu2+ and the pro-oxidative dose-effect of EGCG with Cu2+ were investigated. Experimental results indicated that the carbon quantum dots had pH dependence and good stability behavior. The relative quantum yield was 73.67%. When the concentration ratio between Cu2+ and EGCG was 2:1, the fluorescence quenching of carbon quantum dots was serious, and EGCG had strong pro-oxidative activity. This study provides a theoretical basis for the application of EGCG as a functional substance. |
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| Keywords: | carbon quantum dot fluorescence probe epigallocatechin-3-gallate pro-oxidative activity dose-effect relationship |
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