林蛙油提升雌激素的机制研究

研究林蛙油对正常大鼠和去势大鼠性器官及性激素水平的影响,探讨服用林蛙油提升大鼠血清雌激素的机制。制备去卵巢大鼠,随机分为去势对照组、去势+林蛙油组,正常大鼠随机分为正常对照组、正常+林蛙油组,对正常+林蛙油组和去势+林蛙油组的大鼠每日灌胃200 mg/kg林蛙油,连续灌胃4周。所有大鼠末次灌胃1 h后麻醉,腹主动脉取血,分离血清测定性激素6项指标,取子宫和卵巢(正常大鼠),称量湿重,进行病理组织学检查。结果表明正常大鼠服用林蛙油后,血清雌二醇、睾酮含量显著上升(P<0.05),卵巢病理检查发现,有排卵后黄体增多的现象。去势大鼠服用林蛙油后,血清睾酮、泌乳素含量显著上升(P<0.05),子宫病理检查发现,子宫内膜上皮细胞厚度显著增加(P<0.05)。结论:林蛙油具有一定的外源雌激素作用,但提升服用者雌激素的主要作用机制可能类似于促性腺激素作用机制。

Study on Mechanism of Oviductus Ranae Promoting Estrogen

To research the effect of Oviductus Ranae(OR)on sexual organs and hormone levels in normal and ovariectomized rats and to explore the mechanism of sex hormone promotion. Ovariectomized rats were prepared and randomly divided into castrate control group and castrate+OR group. Normal rats were divided into normal control group and normal+OR group. Rats in castrate+OR group and normal+OR group were given OR by gavage for 4 weeks. All the rats were anaesthetized 1 hour after the last intragastric administraton,and blood was taken from aorta abdominalis. The serum was separated for the determination of the content of 6 indicators of sex hormones. The ovary(normal rats)and uterus were taken for histopathological examination after weighing. The result showed that the serum estradiol and testosterone of normal rats were significantly increased after taking OR(P<0.05),and ovarian pathological examination discovered the phenomenon that had corpus luteum to increase. The serum testosterone and prolactin of castrate rats were significantly increased after taking OR(P<0.05),and uterine pathological examination discovered the thickness of endometrial epithelial cells increased significantly(P<0.05). Conclusion:Oviductus Ranae has some effects of exogenous estrogen,but the main mechanism of promoting estrogen may be similar to that of gonadotropin.