气相色谱法测定氨基酸婴儿配方粉中肌醇的含量

目的建立一种适用于氨基酸婴儿配方粉中肌醇检测的气相色谱方法。方法样品中的肌醇用水和乙醇提取后,与硅烷化试剂衍生,正己烷提取,经气相色谱分离,氢火焰离子化检测器检测,外标法定量。结果优化了硅烷化条件,改进了前处理过程,肌醇衍生物在0～20mg/L范围内线性关系良好,相关系数r~2为0.9995,在3个添加水平的回收率为88.0%～108.0%,相对标准偏差为1.0%～5.4%,方法检出限为1.0 mg/100 g,定量限为3.0 mg/100 g。结论该方法准确可靠,重复性好,适用于氨基酸婴儿配方粉中肌醇含量的测定。

Determination of inositol in free amino acid infant formula by gas chromatography

Objective To determination inositol in free amino acid infant formula by gas chromatography. Methods After the inositol was extracted from the sample with water and ethanol, it was derived from silane reagent, extracted with n-hexane, separated by gas chromatography, detected by hydrogen flame ionization detector, and quantified by external standard method. Results The pretreatment process was improved and the silylation conditions were optimized. The inositol derivatives had a good linear relationship in the concentration range of 0? 20 mg/L with correlation coefficients of 0.9995. The recoveries were 88.0%?108.0% for inositol at 3 spiked levels. The relative standard deviations were 1.0%?5.4%. The limit of detection and limit of quantitation for inositol were 1.0 mg/100 g and 3.0 mg/100 g, respectively. Conclusion The proposed method is accurate, reliable and reproducible, which is suitable for detecting inositol in free amino acid infant formula.