盐析法制备寒富苹果渣果胶及其抗氧化性研究

为使苹果渣变废为宝,利用其生产果胶等高附加值产品,本文以酸法提取寒富苹果果渣中的果胶提取液为原料,采用盐析法得到果胶盐沉淀物,并对该沉淀进行脱盐处理获得果胶物质,期间对相关工艺进行优化,最后进行了果胶的抗氧化性研究。结果表明:硫酸铝为盐析法沉淀果胶的最佳用盐,沉淀果胶的最佳工艺参数为沉淀温度74 ℃、保温时间69 min、pH5.0、料液比1:17 (g:mL),此工艺下果胶得率达15.59%;脱盐最佳工艺为脱盐液中盐酸含量3%、脱盐液用量50 mL每克果胶盐、脱盐时间40 min、脱盐温度40 ℃,所得果胶质量为0.89 g。寒富苹果渣果胶具有清除自由基和抑制脂质过氧化的能力,可作为一种天然的抗氧化剂进行开发。

Study on Pectin from Hanfu Apple Pomace by Salting out Method and the Antioxidation of Pectin

In order to make apple pomace turn waste into treasure and use it to produce high value-added products such as pectin,pectin was extracted from Hanfu apple pomace by acid method. And the pectin salt precipitate was obtained by salting out method,and desalted the precipitate to obtain a pectin substance,and optimized the process flow. Finally,the antioxidant properties of pectin were studied. The results showed that the best salt for the precipitation of pectin by salting out method was aluminum sulfate,the optimum process parameters for precipitating pectin were:Precipitation temperature 74 ℃,holding time 69 min,pH5.0,ratio of material to liquid 1:17 g:mL. Under this process,the yield of pectin reached 15.59%. The best process for desalination was:3% hydrochloric acid content in desalting solution,50 mL of desalting liquid per gram of pectin salt,desalinization time 40 min,desalting temperature 40 ℃. And the obtained pectin mass was 0.89 g. Pectin in Hanfu apple pomace had the ability to scavenge free radicals and inhibit lipid peroxidation,and could be developed as a natural antioxidant.