超声辅助提取-液相色谱-串联质谱法同时测定马卡龙中人工合成色素与天然色素

目的建立超声辅助提取-液相色谱-串联质谱法同时测定马卡龙中的8种人工合成色素与天然色素。方法样品在60%的乙醇水溶液中,使用超声辅助方式提取色素,提取液过滤后直接采用液相色谱-串联质谱法进行检测,基质外标法进行定量。结果在较宽的线性范围内,线性关系良好(r~20.99);在3个添加水平下,回收率在80.3%～103.7%之间,且相对标准偏差均小于15%;8种色素的检出限为0.27～0.84mg/kg,定量限为0.9～2.6 mg/kg。结论该方法快速、简便,灵敏度较高,适用于马卡龙中人工合成色素与天然色素的测定。

Simultaneous determination of synthetic and natural pigments in macaron with ultrasound-assisted extraction-liquid chromatography-electrospray ionization tandem mass spectrometry

Objective To establish a method for simultaneous determination of 8 synthetic and natural pigments in macaron by ultrasound-assisted extraction and liquid chromatography-tandem mass spectrometry. Methods The sample was extracted by ultrasonic assisted method in 60% ethanol aqueous solution. After filtration, the extract was detected by liquid chromatography-tandem mass spectrometry and quantified by matrix external standard method. Results In a wide linear range, the linear relationship was good (r2>0.99); at the 3 levels of addition, the recoveries were between 80.3% and 103.7%, and the relative standard deviations were less than 15%. The limits of detection of 8 pigments were 0.27-0.84 mg/kg, and the limits of quantification were 0.9-2.6 mg/kg. Conclusion The method is rapid, simple and sensitive, which is suitable for the determination of synthetic pigments and natural pigments in macarons.