二步酶解法制备鱼鳞明胶抗氧化肽及其抗氧化活性研究

通过碱性蛋白酶进行第一步酶解,胰蛋白酶、风味蛋白酶、木瓜蛋白酶和胃蛋白酶分别进行第二步酶解制备鱼鳞明胶抗氧化肽。结果表明,二步酶解法能有效提高酶解物的水解度,降低水解物的分子量,碱性蛋白酶-胰蛋白酶水解物具有显著的自由基清除能力和Fe^2+螯合能力。通过响应面优化的胰蛋白酶最佳二步酶解工艺为:底物浓度100mg/mL、pH7.8、温度53℃、时间50min,该条件下制备的水解物的Fe2+螯合率为65.72%,清除DPPH·、·OH和O2^-·的IC50值分别是7.39,0.68,1.84mg/mL。

Preparation of antioxidant peptides by Two-Step enzymatic hydrolysis of fish scale gelatin

Based on the alcalase hydrolysis, the grass carp scales gelatin were subjected to two-step enzymatic hydrolysis with trypsin, flavor protease, papain and pepsin respectively, to prepare antioxidant peptides.Results showed that two-step enzymatic hydrolysis could effectively increase the hydrolysis degree and decrease the molecular weight of hydrolysates.The alcalase-trypsin hydrolysates had significant free radical scavenging ability and Fe^2+ chelating ability.Response surface methodology was performed to optimize the two-step enzymatic hydrolysis conditions, and the optimal enzymatic hydrolysis parameters were as follows: substrate concentration of 100 mg/mL, pH 7.8, hydrolysis temperature of 53 ℃ and hydrolysis time of 50 min.Under this optimal hydrolysis condition, the maximum Fe 2+ chelating ability was 65.72% and the IC 50 value for DPPH·,·OH and O2^-· were 7.39, 0.68 and 1.84 mg/mL, respectively.