粉色、黑色花生种皮色素的抗氧化活性及组分差异分析

为了深入了解粉色和黑色花生种皮色素的抗氧化活性及组分差异,采用高效液相色谱-高分辨飞行时间质谱(UPLC-Q-TOF-MS)联用仪分析了粉色和黑色花生种皮色素的组分,从粉色花生种皮色素中鉴定出(表)儿茶素和2种原花色素二聚体、1种原花色素三聚体和2种原花色素四聚,黑色花生种皮色素中鉴定出原花色素二聚体、三聚体、四聚体和2种矢车菊素、4种飞燕草素类组分。采用分光光度法测定了粉色、黑色花生种皮色素的抗氧化活性,粉色花生种皮色素的抗氧化能力为(17.46±1.88) U/mg,黑色花生种皮色素的总抗氧化能力(23.77±1.58) U/mg;黑色花生种皮色素对羟自由基和超氧阴离子的半抑制力IC₅₀分别为 0.58、1.79 mg/mL;粉色花生种皮色素对羟自由基和超氧阴离子的半抑制力IC₅₀分别为 0.39、1.41 mg/mL。2种不同颜色花生种皮色素的组分具有明显差异,其抗氧化活性也存在异同之处,黑色花生总抗氧化能力强于粉色花生种皮色素,而抑制羟自由基和超氧阴离子的能力弱于粉色花生种皮色素。

Difference Analysis in Antioxidant Activity and Composition of Pigments from Pink and Black Peanut Seed Coat

To have a deep understanding of the difference in antioxidant activity and composition of the seed coat pigments between pink and black peanuts,the composition of seed coat pigments from pink and black peanuts were analyzed by UPLCQ- TOF-MS.Catechin,two dimer,one trimer and two tetramers of proanthocyanidins were identified in the pink peanut coat, while two cyanidin,four delpinidin besides dimer,trimer and tetramers of proanthocyanidins were identified in black peanuts seed coat.The antioxidant activity of pink and black peanut seed coat pigment were determined by spectrophotometry.The results showed that the antioxidant activity of pink peanut seed coat pigments was( 17.46 + 1.88) U/mg.the antioxidant activity of black peanut seed coat pigments was( 23.77 + 1.58) U/mg;the semi- inhibitory effect IC50 of black peanut seed coat pigments on hydroxyl radicals and superoxide anions were 0.58 and 1.79,respectively,and that of red peanut seed coat pigments on hydroxyl Semi- inhibitory IC50 of oxygen anion were 0.39 and 1.41 mg /mL.Its compounds and antioxidant activity had obvious differences.The total antioxidant activity of black peanut was stronger than that of pink peanut,while the ability of inhibiting hydroxyl radical and superoxide anion was weaker than that of pink peanut.