响应面法优化猕猴桃叶绿素提取工艺

以宜昌绿肉猕猴桃果肉为实验材料,采用浸提法,在单因素实验基础上,以OD₆₆₆值为指标,根据Box-Behnken设计,采用4因素3水平响应面分析方法,对叶绿素提取条件进行优化研究,并对各因素(提取剂浓度、液料比、提取时间和提取温度)的显著性和交互作用进行了分析。结果表明:提取时间和提取温度是影响叶绿素提取的极显著因素(p<0.01)。优化所得工艺参数为:提取溶剂乙酸乙酯、液料比1.22:1 mL/g、乙酸乙酯体积分数59.6%、提取时间236 min、提取温度59 ℃、吸光值为0.636±0.01,此时通过Arnon法测得叶绿素含量为27.73 μg/g。

Optimization of Extraction Process of Chlorophyll from Kiwifruit by Response Surface Method

Taking Yichang green kiwifruit pulp as experimental material,using organic solvent extraction,basing on single factor experiments with OD₆₆₆ as an indicator,according to the Box-Behnken design,a 4-factor 3-level response surface analysis method was used to optimize chlorophyll extraction conditions,and the significance and interaction of various factors(concentration of extraction solvent,liquid to material ratio,extraction time,extraction temperature)were analyzed. Results showed that extraction time and extraction temperature were extremly significant factors affecting chlorophyll extraction(p<0.01).The optimized process parameters were as follows:extraction solvent was ethyl acetate,liquid/solid ratio was 1.22:1 mL/g,ethyl acetate volume fraction was 59.6%,extraction time was 236 min,extraction temperature was 59 ℃,absorbance value was 0.636±0.01. At this time,the chlorophyll content was 27.73 μg/g as measured by the Arnon method.