| HPLC-DAD-Q-TOF-MS在线筛选鉴定牛蒡中的抗氧化成分 |
| |
| 引用本文： | 郑振佳,张瑞凌,张敏敏,邱志常,张斌,乔旭光. HPLC-DAD-Q-TOF-MS在线筛选鉴定牛蒡中的抗氧化成分[J]. 食品科学, 2019, 40(8): 175-179. DOI: 10.7506/spkx1002-6630-20180201-014 |
| |
| 作者姓名： | 郑振佳 张瑞凌 张敏敏 邱志常 张斌 乔旭光 |
| |
| 作者单位： | 1.山东农业大学食品科学与工程学院，山东省高校食品加工技术与质量控制重点实验室，山东 泰安 271018；2.齐鲁工业大学（山东省科学院），山东省分析测试中心，山东省中药质量控制技术重点实验室，山东 济南 250014 |
| |
| 基金项目： | 公益性行业（农业）科研专项（201503142）；国家自然科学基金青年科学基金项目（21506119）；郭兰萍泰山学者岗位平台；山东省2017年度农业重大应用技术创新项目 |
| |
| 摘 要： | 建立高效液相色谱-飞行时间质谱联用在线筛选并鉴定牛蒡提取物中抗氧化活性成分的方法。样品经在线色谱柱分离后进行分流，一路与稳定的1,1-二苯基-2-三硝基苯肼-甲醇溶液混合，实现在线筛选自由基清除剂；另一路进入二极管阵列检测器-串联三重四极杆飞行时间质谱，用于化合物的快速鉴别。负离子模式下，通过高分辨率质谱获得的相对分子质量结合化合物的紫外吸收特征，参考相关文献和部分对照品验证，从牛蒡乙酸乙酯相中筛选并鉴定出咖啡酸及衍生物19 种。本方法可实现快速定向筛选和鉴定食品及中药等复杂体系中的抗氧化活性成分。
|
| 关 键 词： | 牛蒡 抗氧化 在线筛选 鉴定 高效液相色谱-二极管阵列检测器-串联三重四极杆飞行时间质谱 |
On-line Screening for and Identification of Antioxidant Compounds from Burdock Roots (Arctium lappa L.) by High Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry |
| |
| ZHENG Zhenjia,ZHANG Ruiling,ZHANG Minmin,QIU Zhichang,ZHANG Bin,QIAO Xuguang. On-line Screening for and Identification of Antioxidant Compounds from Burdock Roots (Arctium lappa L.) by High Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry[J]. Food Science, 2019, 40(8): 175-179. DOI: 10.7506/spkx1002-6630-20180201-014 |
| |
| Authors: | ZHENG Zhenjia ZHANG Ruiling ZHANG Minmin QIU Zhichang ZHANG Bin QIAO Xuguang |
| |
| Affiliation: | 1. Key Laboratory of Food Processing Technology and Quality Control in Shandong Province, College of Food Science and Engineering, Shandong Agricultural University, Tai’an 271018, China; 2. Shandong Analysis and Test Center, Key Laboratory of Traditional Chinese Medicine Quality Control Technology, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China |
| |
| Abstract: | A new method based on high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-DAD-Q-TOF-MS) was developed for the rapid screening for and identification of antioxidant compounds in burdock root extract. The sample was divided into two parts by the first HPLC; one part flowed through the second HPLC with the 1,1-diphenyl-2-picrylhydrazyl (DPPH) solution for on-line screening for the antioxidants; the other was flowed through DAD-Q-TOF-MS for on-line rapid identification. Caffeic acid and its 18 derivatives were identified by their relative molecular masses as determined by high resolution MS in the negative ion mode and by comparison of their UV absorption characteristics with literature data and reference standards. Overall, this method is a powerful tool for rapid screening for and identification of antioxidant compounds from complicated systems such as foods and Chinese traditional medicinal materials. |
| |
| Keywords: | burdock (Arctium lappa L.) antioxidant compounds on-line screening identification HPLC-DAD-Q-TOF-MS |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《食品科学》浏览原始摘要信息 |
|
点击此处可从《食品科学》下载全文 |
