响应面优化超声波-微波协同提取凤眼莲黄酮工艺及其不同部位黄酮抗氧化活性分析

目的:采用Box-Behnken法优化超声波-微波协同提取凤眼莲黄酮工艺,并对其进行抗氧化活性分析。方法:以超声波功率、微波功率、料液比和提取时间为主要影响因素,在单因素实验基础上,以黄酮得率为响应值,利用响应面实验优化凤眼莲黄酮的提取条件。通过对凤眼莲不同部位提取的黄酮进行总还原能力、清除DPPH自由基和清除超氧阴离子自由基能力的测定,对凤眼莲黄酮的抗氧化能力进行评价。结果:最佳提取工艺为超声波功率600 W,微波功率400 W,料液比1∶22 (g/m L),提取时间21 min,在此工艺条件下得到凤眼莲黄酮得率为3.64%±0.07%,回归模型的预测值与真实值相近,模型拟合程度较好。不同部位的黄酮类化合物抗氧化活性大小依次为叶>根>茎,清除DPPH自由基和超氧阴离子自由基的IC50分别为:凤眼莲叶0.569、0.389 mg/m L,凤眼莲根0.754、0.555 mg/m L,凤眼莲茎0.837、0.646 mg/m L。结论:响应面优化超声波-微波协同提取凤眼莲黄酮的工艺条件合理,效率较高,得到的黄酮具有良好的抗氧化能力,可进一步开发利用。

Optimization of Ultrasonic-microwave Synergistic Extraction of Flavonoids from Eichhornia crassipes by Response Surface Methodology and Analysis of Antioxidant Activity of Flavonoids from Its Different Parts

Objective:To optimize the ultrasonic-microwave synergistic extraction process of flavonoids from Eichhornia crassipes by Box-Behnken design,and to analyze antioxidant activity from different parts.Methods:Ultrasonic power,microwave power, material-to-liquid ratios and extraction time were set as the main influencing factors,based on the single factor experiment,the total flavonoid yield was took as the response value,response surface methodology(RSM)was used to optimize the extraction process of total flavonoids from Eichhornia crassipes.By the research of the total reduction ability,DPPH and superoxide anion free radical scavenging abilities to analysis the antioxidant ability of different parts from Eichhornia crassipes.Results:The optimal extraction conditions were determined as follows:Ultrasonic power 600 W,microwave power 400 W,material-to-liquid ratios 1∶ 22 (g/mL),extracting time 21 min.Under these conditions,the yield of flavonoids was 3.64% ±0.07%.The predicted value of the regression model was similar to the real value,and the model fits well.The antioxidant activity of flavonoids in different parts was:Leaf > root > stem,the IC 50 of scavenging DPPH and superoxide anion free radical respectively were:the leaf of Eichhornia crassipes 0.569,0.389 mg/mL,the root of Eichhornia crassipes 0.754,0.555 mg/mL,the stem of Eichhornia crassipes 0.837,0.646 mg/mL.Conclusion:The process conditions of ultrasonic-microwave synergistic extraction of flavonoids from Eichhornia crassipes by response surface methodology optimization were reasonable and had high efficiency.The obtained flavonoid had good antioxidant capacity and could be further developed and utilized.