液相色谱-串联质谱法测定糖果中2-(4-甲氧基苯氧基)-丙酸钠甜味抑制剂

建立了高效液相色谱-串联质谱法检测糖果中的2-(4-甲氧基苯氧基)-丙酸钠的方法。采用水溶液(pH8.0):乙腈=1.5:1 (V/V)作为提取溶剂,然后用正己烷去除油脂,取水层清液过膜,进行检测。采用电喷雾负离子模式(ESI-)和选择反应监测扫描(SRM)模式,外标法定量。结果表明,该方法在6.25~100 μg/L范围内线性关系良好(相关系数r=0.9998),检出限为8.0 μg/kg,定量限为20.0 μg/kg。回收率为82.7%~90.7%,相对标准偏差为2.5%~4.2%。该方法前处理简单、重现性好、灵敏度高,为糖果中的2-(4-甲氧基苯氧基)-丙酸钠的检测提供一种新的选择。

Determination of 2-(4-Methoxyphenoxy)-Propionic Acid Sweetness Inhibitor in Candies by Liquid Chromatography-Tandem Mass Spectrometry

A high performance liquid chromatography-tandem mass spectrometryic(HPLC-MS/MS)method was established for the detection of sodium 2-(4-methoxyphenoxy)-propanoate in candies.The sample was pretreated by using an aqueous solution(pH8.0)∶acetonitrile=1.5∶1(V/V)as an extraction solvent,and then fat was removed by hexane. The clear liquid filtrated with membrane was detected. The analytes were identified by HPLC-MS/MS with the negative electrospray ionization(ESI^-)under selected reaction monitoring(SRM)mode,and analyzed quantitatively by the external standard method. Results showed that the calibration curves were linear in the concentration range of 6.25~100.00 μg/L(correlation coefficients was 0.9998).The limits of detection(LODs)of the sodium 2-(4-methoxyphenoxy)-propanoate was 8.0 μg/kg,and the limits of quantitation(LOQs)was 20.0 μg/kg. The average recoveries were in the range of 82.7%~90.7% with relative standard derivations(RSDs)of 1.2%~2.5%.The method was simple,sensitive and accurate. It provided a new method of the determination of the sodium 2-(4-methoxyphenoxy)-propanoate residue in candies.