双向电泳技术鉴别牛羊乳品真实性

采用双向电泳技术对牛羊乳品的蛋白质指纹进行分析。首先通过全乳图谱分析牛羊乳的差异蛋白，然后针对婴幼儿配方粉、乳清粉等开展方法特异性研究，通过婴幼儿配方粉添加实验开展方法灵敏度研究，最后对市售配方粉、液态乳、酸乳等进行检测。总体上，双向电泳技术信息丰度高，信息直观，重复性良好，能准确分辨牛羊乳及乳清，灵敏度达到5%。通过检测，8 份市售样品中有1 份酸羊乳实际为牛乳，图谱蛋白指纹信息清晰，直观反映产品真实性。

Authentication of Cow and Goat Milk Products by Two-Dimensional Electrophoresis

Two-dimensional gel electrophoresis (2DGE) was applied in protein fingerprinting of cow and goat milk products. First of all, the protein profiles of cow and goat milk were compared to find out the differential proteins. Afterwards, the specificity of this method was tested by applying it to infant formula and whey protein powder from cow and goat milk and the sensitivity was evaluated by using different proportion mixtures of cow milk infant formula and goat milk infant formula. Finally, commercialized infant formula, liquid milk and yoghurt samples were tested. 2DGE method was confirmed to be highly informative and highly repeatable and it could visually present protein profiles. As proved by our data, liquid milk, whey protein and infant formula could be distinguished clearly by 2DGE and the detection limit reached 5%. Of 8 commercialized samples, one labelled as fermented goat milk was identified as cow milk with a clear protein fingerprint which could visually reflect the authenticity of the product