祁白术多酚提取工艺及抗氧化、抑菌活性研究

为研究表面活性剂十二烷基苯磺酸钠(sodium dodecyl sulfonate,SDS)协同超声波提取祁白术多酚的工艺参数,以多酚含量为指标,在单因素试验的基础上,采用响应面法优化提取工艺条件,并对祁白术多酚的体外抗氧化、抑菌活性进行研究。结果表明,祁白术多酚的最优工艺条件为:SDS添加量0.47%,超声波功率150 W,超声温度48℃,乙醇浓度64%,超声时间15 min,料液比1:25(g/mL),该条件下提取的多酚含量高达(38.53±0.51)mg/g。祁白术多酚具有显著的抗氧化活性,其对DPPH、ABTS自由基清除作用的半数抑制浓度(IC₅₀)分别为0.056,0.050mg/mL。祁白术多酚对4种受试菌均有显著的抑制作用,多酚的质量浓度与抑制作用呈量效关系,其对大肠杆菌、金黄色葡萄球菌、绿脓杆菌的最低抑制浓度(MIC)为0.25mg/mL,对枯草芽孢杆菌的MIC为0.50mg/mL。

Study on extraction process of polyphenols from Atractylodes macrocephalaKoidz grown in Qimen and its antioxidant and antibacterial activities

The present study aimed to optimize the process parameters for the surfactant sodium dodecyl sulfonate(SDS)-ultrasonic extraction technology of polyphenols from Atractylodes macrocephala Koidz grown in Qimen(Qi Baizhu).The effects of polyphenols from Qi Baizhu(QBP)on antioxidant and antibacterial activities were explored.The results showed that the optimal process conditions were as follows:SDS concentration 0.47%,extraction temperature 48 ℃,ultrasonic power 150 W,ethanol concentration 64%,extraction time 15 min,and solid-liquid ratio 1:25(g/mL).Under these conditions,the content of QBP was(38.53±0.51)mg/g.QBP exhibited strong DPPH and ABTS free radical scavenging capacities with IC50 values of 0.056,0.050 mg/mL,respectively.QBP also exerted the inhibition effects on four common bacterial strains.There was a good linear dependence between the antibacterial capacity and the concentration.Its minimum inhibitory concentration(MIC)against E.coli,S.aureus and P.aeruginosa was 0.25 mg/mL,while the MIC against B.subtilis was 0.50 mg/mL.