16S rRNA基因序列、生化鉴定、质谱3类方法鉴定常见微生物的结果分析

目的比较16SrRNA基因序列、生化鉴定、质谱鉴定3类实验方法分析沙门氏菌、金黄色葡萄球菌、蜡样芽孢杆菌的鉴定结果的异同。方法挑选沙门氏菌、金黄色葡萄球菌、蜡样芽孢杆菌各50株,分别进行16S rRNA基因序列测定、VITEK COMPACT 2生化鉴定、质谱鉴定3类实验,并比较3类实验的鉴定结果。结果 3类实验方法对大部分沙门氏菌鉴定在属水平,生化鉴定方法对少数血清型鉴定到种水平;对金黄色葡萄球菌均鉴定到种水平; 16SrRNA基因序列、生化方法对蜡样芽孢杆菌鉴定到属水平,质谱鉴定到种水平。结论 3类实验方法对大部分沙门氏菌、所有金黄色葡萄球菌鉴定水平相同,质谱鉴定蜡样芽孢杆菌的结果更准确,且质谱鉴定时效性更高。

Analysis of the results identification of common pathogen using 3 methods including 16S rRNA gene sequencing, biochemical reaction identification and mass spectrum identification

Objective To compare the differences and similarities of results of 3 types of experimental methods, including 16S rRNA gene sequence, VITEK COMPACT 2 biochemical reaction and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) on the determination of Salmonella, Staphylococcus aureus and Bacillus cereus. Methods Fifty strains of Salmonella, Staphylococcus aureus and Bacillus cereus were selected respectively to be identified by 16S rRNA gene sequence, VITEK COMPACT 2 biochemical identification and MALDI-TOF MS, and the identification results of the 3 kinds of experiments were compared. Results Three experimental methods were used to identify most Salmonella at the genus level. And VITEK COMPACT 2 biochemical reaction identified a few serotypes at the species level. The results of Staphylococcus aureus with the above methods were all on species. Bacillus cereus can be on species level with MALDI-TOF MS, but only on genus with the other methods. Conclusion The identification results of most Salmonella and all Staphylococcus aureus are consistent by the 3 methods, but MALDI-TOF MS can identify the Bacillus cereus accurately. MALDI-TOF MS presents higher timeliness on pathogen identification.