沙棘多糖清除自由基及抗脂质过氧化作用研究

目的:探讨沙棘多糖体外清除自由基及抗脂质过氧化作用。方法:采用体外抗氧化活性法测定沙棘多糖对DPPH自由基、羟自由基和超氧阴离子自由基清除能力及沙棘多糖总还原能力;制备5%小鼠肝脏匀浆,通过模拟建立体外小鼠肝匀浆自发性脂质过氧化模型、CCl₄体外诱导小鼠肝匀浆脂质过氧化模型、H₂O₂体外诱导小鼠肝匀浆脂质过氧化模型、Fe2+-V_C 体外诱导小鼠肝脏脂质过氧化模型,利用TBA显色法,观察沙棘多糖对脂质过氧化的抑制作用。结果:沙棘多糖对DPPH自由基、OH自由基和超氧阴离子自由基都具有一定的清除能力,其IC₅₀值分别为1.55、1.23、8.31 mg/mL,其浓度为2 mg/mL时,还原能力稍高于同浓度V_C。沙棘多糖对小鼠肝匀浆自发性脂质过氧化及CCl₄、H₂O₂、Fe2+-V_C所诱导的肝脏脂质过氧化均具有抑制作用,其IC₅₀值分别为1.10、1.59、9.13、1.39 mg/mL。结论:沙棘多糖具有一定的抗氧化活性及抗脂质过氧化作用。

Study on Free Radical Scavenging and Anti-lipid Peroxidation of Seabuckthorn Polysaccharide

Objective:To investigate the scavenging effects of seabuckthorn polysaccharides on free radicals and anti-lipid peroxidation in vitro. Methods:The scavenging ability of seabuckthorn polysaccharides to DPPH free radicals,hydroxyl radicals·(OH)and superoxide anion(O₂-·)and the total reducing ability of seabuckthorn polysaccharides were determined by in vitro antioxidant activity method. Sulphur,model of spontaneous lipid peroxidation of mouse liver homogenate in vitro,model of lipid peroxidation induced by CCl₄ in mouse liver homogenate,H₂O₂ in vitro induction of lipid peroxidation model of mouse liver homogenate,induction of lipid peroxidation in mouse liver by Fe2+-V_C in vitro,and the inhibition effect of seabuckthorn polysaccharide on lipid peroxidation was observed by TBA chromogenic method. Results:Hippophae rhamnoides polysaccharides had certain scavenging ability to DPPH free radicals,·OH free radicals and O₂-·free radicals,and their IC₅₀ values were 1.55,1.23 and 8.31 mg/mL,respectively. At a concentration of 2 mg/mL,the reducing power was slightly higher than the same concentration of V_C. Seabuckthorn polysaccharide inhibited spontaneous lipid peroxidation and liver lipid peroxidation induced by CCl₄,H₂O₂ and Fe2+-V_C in mouse liver homogenate,and its IC₅₀ values were 1.10,1.59,9.13,1.39 mg/mL.Conclusion:Hippophae rhamnoides polysaccharide has certain antioxidant activity and anti-lipid peroxidation.