木犀草素对高血脂症SD大鼠肝脏脂肪变性及抗氧化水平的影响

目的:研究木犀草素对高脂血症SD大鼠肝脏脂肪变性及抗氧化应激的作用。方法:40只雄性SD大鼠,随机分为四组(正常组、模型对照组、木犀草素组、辛伐他汀组)。正常组给予正常饮食,其余各组喂养高脂饮食建立高脂模型,造模成功后分别进行灌胃干预。干预6周后,测体重、肝湿重和肝系数,股动脉取血测定血清总胆固醇(total cholesterol,TC)、甘油三酯(triglycerides,TG)、高密度脂蛋白(high density lipoproteins,HDL)、低密度脂蛋白(low density lipoproteins,LDL),测定肝组织的抗氧化指标:超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、丙二醛(malondialdehyde,MDA)、过氧化氢酶(catalase,CAT),结合病理切片影像和组织学评分观察脂肪变性程度。结果:与正常组相比,模型对照组的体重、肝湿重及肝系数极显著升高(p<0.01),血清TC、TG、LDL-C、HDL-C含量显著升高(p<0.05),光镜下出现明显的脂肪变性特征,脂肪变性评分表明有极显著差异(p<0.01),MDA含量极显著升高(p<0.01),CAT含量显著降低(p<0.05);与模型对照组相比,木犀草素组和辛伐他汀组的体重和肝系数极显著降低(p<0.01),血清TC、TG、LDL-C含量均显著降低(p<0.01,p<0.01,p<0.05),肝细胞脂肪沉积减轻,脂肪变性评分降低,但无显著性差异,抗氧化指标MDA含量极显著降低(p<0.01),CAT含量显著升高(p<0.05,p<0.01)。结论:木犀草素能够明显减轻肝脏脂肪变性,可能是通过降低SD大鼠的血脂水平以及抗氧化应激实现的,木犀草素可能作为降脂和抗氧化的潜在食源性物质。

Effect of Luteolin on Liver Fatty Degeneration and Antioxidation in Hyperlipemia SD Rats

Objective:To study the effect of luteolin on hepatic steatosis and antioxidation in hyperlipidemia rats. Methods:Forty SD rats were randomly divided into normal group,model group,luteolin group and simvastatin group. High-fat diets were fed to establish hyperlipidemia rat model and then given by gastrogavage. Body weight were measured weekly,liver wet weight were measured after 6 weeks,serum TC,TG,HDL,LDL were detected. Antioxidant indicators of liver tissue such as SOD,GSH-Px,MDA,CAT of liver tissue were detected,the degree of steatosis was observed by pathological section and histological score. Results:Compared with the normal group,the body weight,liver weight and liver index of the model control group significantly increased(p<0.01),and TC,TG,LDL-C and HDL-C levels of serum significantly increased(p<0.05),obvious fatty degeneration occurred,MDA content increased significantly(p<0.01),CAT content decreased significantly(p<0.05). Compared with the model control group,the body weight and liver index of the luteolin group and the simvastatin group significantly decreased(p<0.01),and TC,TG,LDL-C levels of serum were significantly decreased(p<0.01,p<0.01,p<0.05). The fatty deposition of liver cells could also be alleviated,the MDA content significantly decreased(p<0.01),and the CAT content significantly increased(p<0.05,p<0.01).Conclusion:Luteolin could significantly reduce liver steatosis,which might be achieved by reducing blood lipid levels in SD rats and combating oxidative stress.