日粮添加姜黄素对IUGR鼠肌肉抗氧化功能及糖代谢的影响

本试验研究日粮添加姜黄素对宫内发育迟缓(intrauterine growth retardation,IUGR)鼠肌肉抗氧化功能和糖代谢的调节作用。试验采用2×2因子设计,采用日粮限饲的方法构建IUGR鼠模型。21日龄断奶后,分别选取12只正常和12只IUGR小鼠随机均分为正常组(NBW)、姜黄素组(NC)和IUGR组、IUGR姜黄素组(IC)。NBW和IUGR组饲喂基础日粮,NC和IC组饲喂基础日粮+400 mg/kg姜黄素。试验饲喂至12周龄,取骨骼肌检测抗氧化和糖代谢酶活力以及相关基因的表达。结果表明:与NBW组相比,IUGR组大鼠肌肉糖酵解潜值(glycolytic potential,GP)和乳酸含量显著升高(p<0.05),总还原型谷胱甘肽(total glutathione,T-GSH)含量、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)和过氧化氢酶(catalase,CAT)活力显著降低(p<0.05);日粮添加姜黄素可显著降低IUGR大鼠肌肉乳酸脱氢酶活性(p<0.05),显著升高(p<0.05)总超氧化物歧化酶活力、CAT活力、糖原含量、琥珀酸脱氢酶活性以及GP。与NBW组相比,IUGR组大鼠肌肉核因子NF-E2相关因子2(nuclear factor erythroid-2 related factor 2,Nrf2)、超氧化物歧化酶1(superoxide dismutase,SOD1)mRNA表达水平均显著降低(p<0.05)。日粮添加姜黄素可显著提高IUGR组大鼠肌肉Nrf2、SOD1 mRNA的表达水平(p<0.05),显著降低腺苷一磷酸依赖的蛋白激酶(adenosine monophosphate-activated protein kinase α1,AMPKα1)和糖原合成酶(glycogen synthase,GS)mRNA的表达水平(p<0.05)。结论:IUGR鼠肌肉抗氧化能力降低,糖原储存能力下降。姜黄素可能通过调节Nrf2、AMPKα1及其他相关基因的表达,来提高IUGR大鼠肌肉抗氧化能力和抑制糖原分解的作用。

Effects of Dietary Curcumin on Anti-oxidation Capacity and Glucose Metabolism in the Muscle of IUGR Rats

The present study was designed to evaluate the effects of dietary supplementation of curcumin on anti-oxidation and glucose metabolism in the muscle of IUGR(intrauterine growth retardation)rats. Twelve normal and twelve IUGR newborn rats were randomly assigned to the following four experimental groups:NBW group(normal rats,fed with basic diets),NC group(normal rats,fed with basic diets+400 mg/kg curcumin),IC group(IUGR rats,fed with basic diets)and IC group(IUGR rats,fed with basic diets+400 mg/kg curcumin). Rats were fed to 12 weeks of age,including a one-week adaptive phase for weaning. Results:Compared with NBW group,IUGR rats had a higher content of LC and GP(glycolytic potential)in the muscle(p<0.05)but had a lower content of T-GSH. Furthermore,the activities of GSH-PX and CAT(catalase)significantly decreased in muscle of the IUGR rats(p<0.05)as compared with those of NBW group. Curcumin significantly reduced the activities of LDH(p<0.05),whereas increased the activities of T-SOD、CAT and SDH in the muscle of IUGR rats(p<0.05),the content of GLY and GP also increased. Compared with NBW group,the mRNA expression of Nrf2(nuclear factor erythroid-2 related factor 2)and SOD1(superoxide dismutase 1)in the muscle of IUGR rats were significantly down-regulated(p<0.05).However,curcumin up-regulated the mRNA expressions of Nrf2 and SOD1(p<0.05),whereas down-regulated the mRNA expressions of AMPKα1(Adenosine monophosphate-activated protein kinase α1)and GS(glycogen synthase). Conclusions:The anti-oxidant capacity and the glycogen storage capacity of IUGR rats decreased. Curcumin could increase the antioxidant capacity of muscle and inhibit the breakdown of glycogen in IUGR rats by regulating the expression of Nrf2,AMPKα1 and other related genes.