黑果腺肋花楸挥发油化学成分及总黄酮含量分析研究

目的研究黑果腺肋花楸挥发性化学成分的组成及质量分数,分析其总黄酮含量。方法采用超声波提取法提取黑果腺肋花楸果实中的挥发油和总黄酮,挥发油的化学成分通过气相色谱-质谱法(gas chromatography-mass spectrometer, GC-MS)进行分析鉴定,各挥发性化学成分的质量分数采用色谱峰面积归一化法计算。以芦丁为标准品,用紫外可见分光光度仪进行总黄酮含量的分析测定。结果黑果腺肋花楸挥发油经GC-MS分析,共鉴定出36个挥发性化学成分,占挥发油色谱总峰面积的84.14%。芦丁标准品呈现良好线性关系(r~2=0.9997),总黄酮类化合物的平均含量为0.233%,标准偏差(standard deviation,SD)为0.01291,相对标准偏差(relative standard deviation, RSD)为5.53%。结论黑果腺肋花楸中的挥发性成分以烷烃类、酯类、羧酸类、醇类几类化合物为主,其果实中总黄酮类化合物的含量较低。

Analysis of chemical constituents of volatile oils and the contents of total flavonoids in Aronia melanocarpa Elliott

Objective To study the composition and mass fraction of volatile oils in Aronia melanocarpa Elliott, and analyze the total flavonoids contents. Methods The volatile oils and total flavonoids in the fruit of the black fruit gland were extracted by ultrasonic extraction. The chemical constituents of the volatile oils were analyzed by gas chromatography-mass spectrometry (GC-MS). The mass fraction of the components was calculated using the chromatographic peak area normalization method. Total flavonoids contents were detected of by UV-visible spectrophotometer with rutin as standard. Results Thirty-six volatile compounds of the volatile oils from Aronia melanocarpa Elliott were analyzed and identified by GC-MS, which accounted for 84.14% of the total peak area of volatile oils. The rutin standard showed a good linear relationship (r2=0.9997), the average content of total flavonoids was 0.233%, and the standard deviation (SD) was 0.01291. The relative standard deviation (RSD) was 5.53%. Conclusion The volatile components in Aronia melanocarpa Elliott are mainly composed of alkanes, esters, carboxylic acids and alcohols, and the contents of total flavonoids in the fruits are lower.