| 高效液相色谱-蒸发光散射法测定保健食品中 磷脂酰丝氨酸的方法优化 |
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| 引用本文： | 黄兴民,郑家概,张 飞,杨 熙,高 涛,吴 斌,卢嘉豪,邓雨阳. 高效液相色谱-蒸发光散射法测定保健食品中 磷脂酰丝氨酸的方法优化[J]. 食品安全质量检测学报, 2019, 10(12): 3806-3810 |
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| 作者姓名： | 黄兴民 郑家概 张 飞 杨 熙 高 涛 吴 斌 卢嘉豪 邓雨阳 |
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| 作者单位： | 中国广州分析测试中心广东省化学危害应急检测技术重点实验室 |
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| 基金项目： | 广东省科学院发展专项资金项目 (2019GDASYL-0105021)、广东省科学院科技创新专项(2019GDASYL-0302004)、广东省自然科学基金项目(2015A030310466) |
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| 摘 要： | 目的优化高效液相色谱-蒸发光散射法测定保健食品中磷脂酰丝氨酸含量的分析方法。方法以氯仿-甲醇溶液(9:1, V:V)为提取剂,样品经旋涡混合辅助超声提取后高效液相色谱仪测定。以硅胶柱(4.6 mm×250 mm,5μm)作为分离柱,流动相为异丙醇:水:乙酸:三乙胺(84:16:1.5:0.08, V:V:V:V),流速1.0 mL/min,等度洗脱,柱温40℃。蒸发光散射检测器选择不分流模式,漂移管温度100℃,载气(氮气)流速为2.0 L/min,增益值为200。结果磷脂酰丝氨酸在20～320mg/L浓度范围内现呈良好线性关系,相关系数为0.9999,加标回收率为95.7%～100.7%,检出限为4.0 mg/100 g,定量限为11 mg/100 g。采用该方法对14种保健食品中磷脂酰丝氨酸的含量进行测定,结果符合产品质量标准。结论该方法适用范围广,有机溶剂用量少,数据准确可靠和重现性好,可满足实验要求。
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| 关 键 词： | 磷脂酰丝氨酸 旋涡混合辅助超声提取法 高效液相色谱法 蒸发光散射检测器 |
| 收稿时间： | 2019-02-25 |
| 修稿时间： | 2019-06-14 |
Optimization of determination of phosphatidylserine in health foods by high performance liquid chromatography-evaporative light scattering detection |
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| HUANG Xing-Min,ZHENG Jia-Gai,ZHANG Fei,YANG Xi,GAO Tao,WU Bin,LU Jia-Hao and DENG Yu-Yang. Optimization of determination of phosphatidylserine in health foods by high performance liquid chromatography-evaporative light scattering detection[J]. Journal of Food Safety & Quality, 2019, 10(12): 3806-3810 |
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| Authors: | HUANG Xing-Min ZHENG Jia-Gai ZHANG Fei YANG Xi GAO Tao WU Bin LU Jia-Hao DENG Yu-Yang |
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| Affiliation: | Guangdong Provincial Key Laboratory of Emergency Test for Dangerous Chemicals, China National Analytical Center (Guangzhou),Guangdong Provincial Key Laboratory of Emergency Test for Dangerous Chemicals, China National Analytical Center (Guangzhou),Guangdong Provincial Key Laboratory of Emergency Test for Dangerous Chemicals, China National Analytical Center (Guangzhou),Guangdong Provincial Key Laboratory of Emergency Test for Dangerous Chemicals, China National Analytical Center (Guangzhou),Guangdong Provincial Key Laboratory of Emergency Test for Dangerous Chemicals, China National Analytical Center (Guangzhou),Guangdong Provincial Key Laboratory of Emergency Test for Dangerous Chemicals, China National Analytical Center (Guangzhou),Guangdong Provincial Key Laboratory of Emergency Test for Dangerous Chemicals, China National Analytical Center (Guangzhou) and Guangdong Provincial Key Laboratory of Emergency Test for Dangerous Chemicals, China National Analytical Center (Guangzhou) |
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| Abstract: | |
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| Keywords: | phosphatidylserine vortex mixing coupled with ultrasonic extraction high performance liquid chromatography evaporative light scattering detector |
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