| 高效液相色谱法测定大豆磷脂类保健食品中磷脂酰胆碱含量 |
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| 引用本文： | 刘彬丽,唐亚利,金绍明,宁 霄,曹 进.高效液相色谱法测定大豆磷脂类保健食品中磷脂酰胆碱含量[J].食品安全质量检测技术,2019,10(1):90-94. |
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| 作者姓名： | 刘彬丽 唐亚利 金绍明 宁 霄 曹 进 |
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| 作者单位： | 中国食品药品检定研究院 |
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| 摘 要： | 目的建立高效液相色谱法(highperformance liquid chromatography,HPLC)测定大豆磷脂类保健食品中磷脂酰胆碱含量的分析方法。方法采用高效液相色谱仪、Kromasil 100-5-SIL硅胶柱,以流动相正己烷:异丙醇:水=0.25:4:1(V:V:V),流速为0.5 mL/min,检测波长为207 nm,柱温为30℃,进样量10μL,进行检测。结果磷脂酰胆碱浓度为0.1～2.0mg/mL时,线性关系良好,回归方程的相关系数r2=0.9998,相对标准偏差(relative,standarddeviation,RSD)为0.50%,平均回收率为98.01%～98.73%。结论该方法高效,准确,简便,适合于检测大豆磷脂类保健食品中磷脂酰胆碱的含量。
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| 关 键 词： | 大豆磷脂 磷脂酰胆碱 保健食品 高效液相色谱法 |
| 收稿时间： | 2018/10/17 0:00:00 |
| 修稿时间： | 2018/12/21 0:00:00 |
Determination of phosphatidylcholine from soybean phospholipid health products by high performance liquid chromatography |
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| LIU Bin-Li,TANG Ya-Li,JIN Shao-Ming,NING Xiao and CAO Jin.Determination of phosphatidylcholine from soybean phospholipid health products by high performance liquid chromatography[J].Food Safety and Quality Detection Technology,2019,10(1):90-94. |
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| Authors: | LIU Bin-Li TANG Ya-Li JIN Shao-Ming NING Xiao and CAO Jin |
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| Affiliation: | National Institutes for Food and Drug Control,National Institutes for Food and Drug Control,National Institutes for Food and Drug Control,National Institutes for Food and Drug Control and National Institutes for Food and Drug Control |
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| Abstract: | |
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| Keywords: | soybean phospholipids phosphatidylcholine health food high-performance liquid chromatography method |
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