冰藏联用ClO2保鲜对大黄鱼肌肉优势腐败菌及品质的影响

为了保持养殖大黄鱼肌肉质量,延长货架期,本试验采用冷藏保鲜、冰藏保鲜、冰藏联用ClO2保鲜等三种保鲜方式对鲜黄鱼进行处理。以大黄鱼肌肉的微生物变化和品质特性作为评价指标,并通过宏基因组高通量测序技术分析了经上述三种方法处理后大黄鱼肌肉优势菌属的变化。结果表明:冷藏保鲜和冰藏保鲜的菌落总数和TVB-N值随着贮存天数的增加而增大,而冰藏联用ClO2保鲜相对于其他处理方式变化幅度最小;三种处理组的硬度、弹性和咀嚼性均先上升后下降,而pH呈先下降后上升的变化,以冰藏联用ClO2保鲜组的变化幅度最小;冰藏联用ClO2保鲜处理组在贮藏前期脂肪的氧化程度高于其他两组,贮藏5d后差异显著(p<0.05);通过电子鼻对大黄鱼肌肉风味分析表明冰藏联用ClO2保鲜组对其风味保持效果较好;高通量测序结果显示,三个处理组的优势菌属均为希瓦氏菌属、假单胞菌属,但菌群结构有所差别,冰藏联用ClO2可以减少大黄鱼肌肉菌属的种类。

Effect of Ice Storage Combined with ClO2 Preservation on Dominant Spoilage Bacteria and Quality of Large Yellow Croaker Muscle

In order to maintain the muscle quality of the large yellow croaker and extend the shelf life,the fresh yellow croaker was treated in three fresh-keeping methods,such as cold storage,ice storage,ice storage combined with ClO2 preservation.The microbial changes and quality characteristics of the muscles of large yellow croaker were used as evaluation indicators,and the changes of the dominant genus of the large yellow croaker,which were treated by the above-mentioned three methods,were analyzed by the high - throughput sequencing technology of the macrogenome.The results showed that the total number of colonies and TVB-N increased with the increase of storage days,and the three treatment groups showed an upward trend,while the ice storage combined with ClO2 preservation had the smallest change with respect to other treatment methods;The hardness, elasticity,and chewing property of the three treatment groups first increased and then decreased,while the pH decreased first and then increased,the change of the ClO2 fresh- keeping group was the smallest.In the early stage of storage,the oxidation degree of fat in the ice storage combined with ClO2 preservation group was higher than that of the other two groups.The difference was significant after storage for 5 days( p < 0.05).The muscle flavor analysis of large yellow croaker by electronic nose showed that the ice-cold combined with ClO2 preservation group had better flavor retention.High-throughput sequencing results showed that the dominant genus of the three treatment groups were both Shigella and Pseudomonas,but the structure of the flora was different.The combination of ice and ClO2 could reduce the genus kinds of yellow croaker muscle.