植物乳杆菌活菌片剂产品的功能性与稳定性研究

本研究以一株具有润肠通便功能的植物乳杆菌为研究对象,研究其活菌片剂产品的功能与稳定性,通过检测植物乳杆菌581的生长曲线和终点pH评价其对不同益生元的利用效率,采用小鼠便秘模型评估低聚果糖和植物乳杆菌581制成的片剂产品的功效,通过监测产品在4℃和28℃贮存6个月的活菌数、水分含量和水分活度评价产品稳定性。实验结果表明,低聚果糖是与植物乳杆菌581配伍制成片剂产品的优选益生元。以植物乳杆菌581与低聚果糖为主要功能成分所制备的活菌片剂产品连续7 d灌胃便秘模型小鼠能有效改善小鼠的肠道功能,具有润肠通便的功效。其中,低聚果糖能够促进植物乳杆菌581更好的发挥润肠通便功能。同时,该片剂产品在4℃储藏条件下可有效维持其活菌数达到1010 CFU/g,从而充分发挥该产品的益生功能。

Study on the Functionality and Stability of Lactobacillus plantarum Tablets

In this study,a Lactobacillus plantarum with laxative function was taken as study object,the function and stability of its tablet products were studied. The utilization efficiency of Lactobacillus plantarum 581 to different prebiotics was evaluated by detecting its growth curve and end point pH. Mouse constipation model was used to evaluate the efficacy of tablets made from fructo-oligosaccharide(FOS)and L. plantarum 581.The stability of tablets was evaluated by monitoring the number of viable cells,moisture content and water activity of the tablets stored at 4℃ and 28℃ for 6 months. The experimental results showed that FOS was the best prebiotic for L. plantarum 581.The live bacterial tablets prepared by Lactobacillus plantarum 581 and FOS as main functional components could effectively improve the intestinal function of constipation model mice after 7 days of oral administration. It showed that FOS had a promoting effect with L. plantarum 581 and had the function of improving constipation. Meanwhile,the tablet product could effectively maintain its viable count at 4℃(1010 CFU/g),to bring into playing the functions of the probiotic product.