首页 | 本学科首页   官方微博 | 高级检索  
     

分批补料高密度发酵生产丙酸杆菌素及其结构分析
引用本文:郑丽雪,王家皓,陈志鹏,齐斌,王立梅.分批补料高密度发酵生产丙酸杆菌素及其结构分析[J].食品科学,2019,40(10):129-133.
作者姓名:郑丽雪  王家皓  陈志鹏  齐斌  王立梅
作者单位:常熟理工学院 苏州市食品生物技术重点实验室,发酵工程技术研究中心,江苏 常熟 215500
基金项目:江苏省科技厅重点研发计划项目(BE2017316;BE2017326);常熟市科技发展计划项目(CN201614)
摘    要:采用分批补料高密度发酵的方法提高丙酸杆菌素的抑菌活性。从发酵96 h开始,每隔24 h补加2.5 g/L复合碳源(乳酸钠-葡萄糖质量比3∶1)和复合氮源(酵母溶液-玉米酒糟清液体积比1∶30)各100 mL,连续补加5 次后,丙酸杆菌素的抑菌活性从17.6 AU/mL提高到31.2 AU/mL。本研究创新性地将玉米酒糟清液作为发酵氮源代替部分酵母溶液,大大降低了丙酸杆菌素的生产成本。另外,通过红外光谱及蛋白酶敏感实验发现丙酸杆菌素是一种具有抑菌活性的蛋白类物质。本研究结果为丙酸杆菌素的工业化生产及其应用提供一定理论支持。

关 键 词:高密度发酵  分批补料  玉米酒糟清液  丙酸杆菌素  

Production and Structural Analysis of Propionin by High Cell Density Fed-Batch Fermentation
ZHENG Lixue,WANG Jiahao,CHEN Zhipeng,QI Bin,WANG Limei.Production and Structural Analysis of Propionin by High Cell Density Fed-Batch Fermentation[J].Food Science,2019,40(10):129-133.
Authors:ZHENG Lixue  WANG Jiahao  CHEN Zhipeng  QI Bin  WANG Limei
Affiliation:Suzhou Key Laboratory of Food and Biotechnology, Research Center of Fermentation Engineering, Changshu Institute of Technology, Changshu 215500, China
Abstract:In this work, high cell density fed-batch fermentation was carried out to produce propionin with higher antibacterial activity. Starting from 96 h of fermentation, a mixed carbon source (sodium lactate:glucose = 3:1, m/m) and a mixed nitrogen source (yeast extract:corn distiller’s grains liquid = 1:30, V/V) (100 mL each, 2.5 g/L) were added at 24 h intervals for a total of 120 h. As a result, the antibacterial activity of propionin was increased from 17.6 to 31.2 AU/mL. In this study, we innovatively used corn distiller’s grains liquid as an alternative nitrogen source to partially replace yeast extract in order to greatly reduce the production cost of propionin. In addition, propionin was found to be a proteinaceous material with antibacterial activity as revealed by infrared spectroscopy and protease sensitivity experiments. The results of this study lay the foundation for industrial production and application of propionin.
Keywords:high cell density fermentation  fed-batch feeding  corn distiller’s grains liquid  propionin  
本文献已被 CNKI 等数据库收录!
点击此处可从《食品科学》浏览原始摘要信息
点击此处可从《食品科学》下载全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号