陶瓷膜微滤耦合大孔树脂处理对罗非鱼肉酶解多肽理化性质的影响

本文研究无机陶瓷膜微滤和大孔树脂吸附联合处理对罗非鱼肉酶解多肽组分的影响,采用50 nm陶瓷膜处理及AB-8大孔树脂动态吸附罗非鱼肉酶解多肽,应用国标分析基本成分、高效体积排阻色谱(HPSEC)分析分子量分布、氨基酸分析仪测定氨基酸含量、反相液相分析氨基酸疏水性。结果表明,陶瓷膜微滤处理对酶解多肽的氨基酸组成影响不大,且陶瓷膜微滤处理后酶解多肽中1~2、2~3、3~5、5~8 ku组分的比例分别减少了7.29%、17.13%、33.93%和50.0%,说明陶瓷膜微滤对多肽有截留作用,且分子量越大截留比例越大;AB-8大孔树脂吸附使氨基酸总量降低1.99%,必需氨基酸含量降低5.15%,苦味氨基酸与疏水性氨基酸含量分别降低12.35%和8.44%,对2~8 ku多肽的吸附较敏感,对游离氨基酸吸附较少;反相液相分析结果显示,AB-8大孔树脂吸附的洗脱组分含有更多的疏水性肽段。综上所述,AB-8大孔树脂动态吸附可以有效改善罗非鱼肉酶解多肽的苦味。

Effect of Ceramic Membrane Microfiltration Coupled with Macroporous Resin on Hydrolyzed Peptides of Tilapia Meat

The effect of inorganic ceramic membrane microfiltration and macroporous resin adsorption on the physical and chemical properties of the hydrolyzed peptides of tilapia were studied to provide reference for their development and utilization. Dynamic adsorption of tilapia hydrolyzed peptides by 50 nm ceramic membrane and AB-8 macroporous resin. The basic components were analyzed by national standards,and high molecular weight exclusion chromatography(HPSEC)was used to analyze the molecular weight distribution and amino acid analyz for determination of amino acid contents,reverse phase liquid phase analyze for amino acid hydrophobicity. Results showed that the microfiltration treatment of ceramic membrane had little effect on the amino acid composition of enzymatic polypeptides,and the proportion of 1~2,2~3,3~5,5~8 ku components decreased by 7.29%,17.13%,33.93% and 50.0% respectively after the microfiltration treatment of ceramic membrane,which indicated the interception effect of peptides,and the greater the molecular weight,the greater the interception ratio. The adsorption of AB-8 macroporous resin reduced the total amount of amino acids by 1.99%,the content of essential amino acids by 5.15%,and the contents of bitter amino acids and hydrophobic amino acids by 12.35% and 8.44%,respectively. And AB-8 macroporous resin was sensitive to the adsorption of 2~8 ku peptides,and less adsorption for free amino acids. RPLC results showed that the eluted fractions adsorbed by AB-8 macroporous resin contained more hydrophobic peptides. The dynamic adsorption of AB-8 macroporous resin could effectively improve the bitterness of the peptides digested by tilapia.