响应面法优化近江牡蛎多糖多肽联产的酶解工艺

利用响应面法优化近江牡蛎(Ostrea rivularis)多糖多肽联产的酶解工艺条件。通过单因素实验,研究酶解时间、酶配比、料液比对水解度、多肽含量、酸性糖含量以及总糖含量的影响,在此基础上,应用响应面试验建立数学模型,以多肽含量、酸性糖含量和总糖含量为响应值,进行三因素三水平的响应面分析。结果表明,酶解法联产制备近江牡蛎多糖多肽的最佳工艺为时间2.0 h,添加配比为3:1的alcalase和胰蛋白酶(总质量分数为0.8%),料液比1:3.6 g/mL。此条件下进行重复试验,得到近江牡蛎酶解液中多肽含量(251.70±5.73) mg·50 g-1,酸性糖含量(8.17±0.29) mg·50 g-1,总糖含量(380.66±15.14) mg·50 g-1。并按此工艺酶解近江牡蛎全脏器匀浆,得粗多糖21.29%±0.77%,含85.18%±1.00%总糖,20.02%±0.38%酸性糖以及10.84%±0.12%多肽;粗多肽得率为31.41%±0.47%,含84.03%±0.85%多肽,0.11%±0.01%酸性糖以及5.68%±0.15%总糖。与回归方程预测值相近,说明近江牡蛎多糖多肽联产工艺是可行的。

Optimization of Enzymolysis Process for Co-production of Polysaccharides and Polypeptides from Ostrea rivularis by Response Surface Methodology

The optimal enzymolysis conditions of simultaneous extraction of polysaccharides and polypeptides were explored from Ostrea rivularis by response surface methodology.Single factors experiments were used to find out the effects of hydrolysis time, enzymolysis proportion and solid-liquid ratio on the degree of hydrolysis, polypeptide content, glycosaminoglycan content and total-sugar content. Then three mathematical models were set up by response surface experiment. With three response values including polypeptide content, glycosaminoglycan content and total-sugar content, the three-factor and three-level response surface analysis was set up. The results showed that the optimal process for the preparation of polysaccharides from Oujiang oysters by enzymatic hydrolysis was 2.0 h, adding 3:1 alcalase and trypsin (total mass fraction of 0.8%), and the ratio of material to liquid was 1:3.6 g/mL. Under these conditions, repeated experiments were carried out to determine that the content of polypeptides was (251.70±5.73) mg·50 g-1, the content of glycosaminoglycans was (8.17±0.29) mg·50 g-1 and the content of total-sugar was (380.66±15.14) mg·50 g-1.The yield of rough polysaccharides in whole organ of Ostrea rivularis was 21.29±0.77%, which contained 85.15%±1.00% total-sugar, 20.02%±0.38% glycosaminoglycans and 10.84%±0.12%polypeptides.Otherwise the yield of rough polypeptides was 31.41%±0.47%, which contained 84.03%±0.85% polypeptides, 0.11%±0.01% glycosaminoglycans and 5.68%±0.15% total-sugar.In one word, the co-production process for polysaccharides and polypeptides from Ostrea rivularis was feasible, basically the same as the predicted value.