山楂黄酮对小鼠脾淋巴细胞的免疫调节作用

为研究山楂黄酮对小鼠脾淋巴细胞的免疫调节作用,将小鼠分为空白组、左旋咪唑组和不同浓度山楂黄酮处理组(100、200、400和800 μg/mL)。利用MTT法检测山楂黄酮对淋巴细胞增殖的影响,ELISA法检测山楂黄酮对淋巴细胞白细胞介素-6(IL-6)、白细胞介素-4(IL-4)、干扰素-γ(IFN-γ)的影响,流式细胞术检测山楂黄酮对淋巴细胞膜表面标志物(CD4+和CD8+)的影响。结果表明,山楂黄酮在100~400 μg/mL对淋巴细胞增殖和淋巴细胞因子的分泌具有浓度依赖性,IL-6和IFN-γ的分泌极显著高于空白组(P<0.01),IL-4的分泌极显著低于空白组(P<0.01),且400 μg/mL处理组与左旋咪唑组无统计学意义(P>0.05);对淋巴细胞亚群CD4+CD8-、CD4-CD8+具有浓度依赖性的促进作用,且极显著高于空白组、左旋咪唑组(P<0.01);对淋巴细胞亚群CD4+/CD8+百分比极显著高于空白组、左旋咪唑组(P<0.01),200 μg/mL浓度组CD4+/CD8+的比值极显著高于其它浓度组(P<0.01)。100~400 μg/mL山楂黄酮通过诱导淋巴细胞增殖和淋巴细胞因子IL-6、IL-4和IFN-γ的分泌,提高淋巴细胞亚群CD4+/CD8+比值,从而发挥免疫调节作用。

Immunoregulatory Effect of Hawthorn Flavonoids on Spleen Lymphocytes in Mice

To study the immunomodulatory effect of hawthorn flavonoids on spleen lymphocytes in mice,mice were divided into blank group,levamisole group and hawthorn flavonoids treatment group(100,200,400 and 800 μg/mL). MTT method was used to detect the effect of hawthorn flavonoids on lymphocyte proliferation. ELISA method was used to detect the effect of hawthorn flavonoids on lymphocyte interleukin-6(IL-6),interleukin-4(IL-4),interferon-γ(IFN-γ). Flow cytometry was used to detect the effect of hawthorn flavonoids on lymphocyte membrane surface markers(CD4+ and CD8+). Results showed that hawthorn flavonoids had concentration dependence on lymphocyte proliferation and lymphocyte factor secretion ranged from 100 to 400 μg/mL. The secretion of IL-6 and IFN-γ were higher than that of the blank group extremely significant(P<0.01),and the secretion of IL-4 was lower than that of the blank group extremely significant(P<0.01),and there was no statistical significance between the 400 μg/mL treatment group and levamisole group(P>0.05). The percentage of CD4+ CD8- and CD4- CD8+ in lymphocyte subsets had a centration-dependent promoting effect,which was higher than that in blank group and levamisole group extremely significant(P<0.01). The percentage of CD4+/CD8+ in lymphocyte subsets was higher than that in blank group and levamisole group extremely significant(P<0.01),and the ratio of CD4+/CD8+ in 200 μg/mL treatment group was higher than that in other concentration groups(P<0.01). Hawthorn flavone could enhance the CD4+/CD8+ ratio of lymphocyte subpopulation by inducing lymphocyte proliferation and secretion of lymphocyte factors IL-6,IL-4 and IFN-γ ranged from 100 to 400 μg/mL,and it played an immunomodulatory role.