响应面法优化超声辅助提取酸枣仁异牡荆素工艺

本研究旨在用响应面方法优化酸枣仁异牡荆素的超声辅助提取工艺。通过单因素实验考察超声时间、超声功率、乙醇体积分数、超声温度和液固比对异牡荆素得率的影响,以这些因素为自变量,异牡荆素得率为响应值,设计四因素三水平Box-Behnken响应面试验方法。结果表明,响应面法优化超声提取条件:超声功率419 W,液固比16∶1 mL/g,超声时间61 min,乙醇体积分数73.70%,各因素的影响重要性依次为:乙醇体积分数>超声时间>液固比>超声功率。预测最佳得率0.236%与实际得率0.241%相比,相对误差为2.075%,证明模型理论预测值与实际值拟合效果良好。本研究优化了提取异牡荆素的超声辅助技术,为超声辅助提取黄酮类化合物提供了方法借鉴。

Optimization of Ultrasonic Assisted Extraction of Isovitexin from Zizyphi spinosae Semen by Response Surface Methodology

The ultrasonic assisted extraction of isovitexin from Zizyphi spinosae semen was optimized. On the basis of single factor experiment,ultrasound time,ultrasound power,ethanol volume fraction and liquid-solid ratio were selected as independent variables,the yield of isovitexin was used as the response value. The 4 factors and 3 level response surface tests were designed by Box-Behnken experiment. The results showed that the ultrasonic extraction conditions of optimization by response surface methodology was as follows:Ultrasound power 419 W,liquid-solid ratio 16∶1 mL/g,ultrasound time 61 min,ethanol volume fraction 73.70%. The influence factors on the yield of isovitexin was as follows:Ethanol volume fraction>ultrasound time>liquid-solid ratio>ultrasound power. The relative error between the predicted optimum yield 0.236% and the actual yield 0.241% was 2.075%,which proved that the theoretical predicted value of the model fitted well with the actual value. In this study,the ultrasound assisted extraction technology of isovitexin was optimized to provide a reference for ultrasonic-assisted extraction of flavonoids.