超高压处理对养殖大黄鱼肌动球蛋白生化特性的影响

以养殖大黄鱼作为研究对象,研究超高压处理对养殖大黄鱼的肌动球蛋白特性影响。研究用不同压力(300、350、400 MPa)、不同保压时间(3、6、9 min)对养殖大黄鱼肌动球蛋白的钙离子ATP酶、表面疏水性、活性疏基含量、热稳定性、SDS-PAGE电泳五个指标的影响。结果表明:超高压会引起大黄鱼肌动球蛋白随着压力上升和保压时间增加,表面疏水性随之增大,活性巯基含量增加,蛋白质分子量有所下降,结构发生改变。蛋白质热变性变性焓值随保压时间增加而减少,钙离子ATP酶活性在400 MPa整体伴随时间增加呈下降趋势,300和350 MPa伴随时间增加呈现先增后减趋势。分析说明肌动球蛋白受超高压影响,并且影响显著,会致使蛋白结构聚集或断裂,进而影响生化特性。

Effects of Ultra High Pressure Treatment on Biochemical Characteristics of Actin in Cultured Pseudosciaena crocea

Taking cultured large yellow croaker as the reasearch object,the effects of ultrahigh pressure(UHP)treatment on the actin characteristics of cultured large yellow croaker were studied.The effects of different pressure(300,350,400 MPa)and holding time(3,6,9 min)on calcium ion ATPase,surface hydrophobicity,active sparse group content,thermal stability and SDS-PAGE electrophoresis of actin in cultured Pseudosciaena crocea were studied. The results showed that ultra-high pressure could lead to the increase of surface hydrophobicity,the increase of active sulfhydryl content,the decrease of protein molecular weight and the change of structure of actin in large yellow croaker with the increase of pressure and holding time.The value of the denaturation and denaturation of the protein thermal denaturation decreased with the increase of the pressure holding time,and the activity of the calcium ion ATPase decreased with the increase of the time of the whole of 400 MPa,and the concomitant time of 300 MPa and 350 MPa was first increased and then decreased.The results showed that actin was affected by ultrahigh pressure and had a significant effect,which would lead to the aggregation or breakage of protein structure,and then affect the biochemical characteristics.