实时荧光PCR法鉴别玛咖及其掺假物芜菁

为建立玛咖原料及其制品中玛咖及其掺假物芜菁物种鉴别方法,根据defensin基因序列,分别设计了玛咖、芜菁的特异引物探针,建立了两种植物源性成分的实时荧光PCR检测方法。以玛咖、芜菁等33份样品DNA为模板,经实时荧光PCR扩增,结果表明:已建立方法分别能特异扩增玛咖、芜菁成分;灵敏度测试结果表明:本方法检测玛咖、芜菁成分的绝对灵敏度均达0.01 ng/μL,相对灵敏度均达0.1%(w/w),且能准确鉴别市售玛咖制品中的玛咖与芜菁成分。由此说明所建立方法可作为检测玛咖原料及其制品中玛咖与掺假物芜菁成分的特异性鉴别方法。

Identification of Lepidium meyenii Walp (Maca)and Adulterated Brassica rapa L. (Rappini) by Real-time PCR

By the defensin gene,specific primers and probes of maca and rappini were designed,and the real-time fluorescence PCR methods for detection of both botanical components were established.The specificity of both methods were verified by using 33 samples DNA,including maca,rappini,and so on.Sensitivity test results showed that the absolute sensitivity for detecting both components reached 0.01 ng/L,the relative sensitivity was 0.1%(w/w),and the methods could accurately identify maca and rappini components in commercial maca products. Above all,the established methods could be used for the specific identification of maca and its adulterated component rappini in maca raw materials and its products.