海藻酸钠包埋法固定硝基还原假单胞菌的研究

目的优化海藻酸钠和CaCl_2对硝基还原假单胞菌SP.001细胞的固定化条件。方法以海藻酸钠为载体,通过包埋法固定化谷氨酰胺酶,通过单因素实验和正交实验对固定化细胞制备条件进行优化。结果单因素实验中,当海藻酸钠和CaCl_2的浓度分别为4%和3%、酶液量与海藻酸钠的体积比为1:2、固定化时间为3 h时,包埋效果最好,测得固定化细胞回收率较高,可达80.5%。通过正交实验得出最佳组合为:酶液量与海藻酸钠体积比1:3,固定化时间3 h,海藻酸钠浓度4%,CaCl_2浓度3%,固定化细胞回收率达到85.78%。结论本研究优化了海藻酸钠包埋法固定硝基还原假单胞菌的条件,并对固定化细胞的性质进行探讨,为硝基还原假单胞菌所产的谷氨酰胺酶的固定化和应用提供参考。

Study on immobilization of nitro-reducing Pseudomonas by sodium alginate embedding

Objective To optimize the immobilization conditions of sodium alginate and CaCl2 on nitro-reduced Pseudomonas SP.001 cells. Methods Using sodium alginate as carrier, glutaminase was immobilized by embedding method, and the preparation conditions of immobilized cells were optimized by single factor experiment and orthogonal experiment. Results In the single factor experiment, when the concentration of sodium alginate and CaCl2 were 4% and 3%, the volume ratio of enzyme liquid volume to sodium alginate was 1:2, and the immobilization time was 3 h, the embedding effect was the best, and the recovery rate of immobilized cells was high, reaching 80.5%. Through orthogonal test, the best combination was obtained as follows: the volume ratio of enzyme liquid volume to sodium alginate was 1:3, the immobilization time was 3 h, the concentration of sodium alginate was 4%, the concentration of CaCl2 was 3%, and the recovery rate of immobilized cells reached 85.78%. Conclusion This study optimizes the conditions for the immobilization of nitroreductive pseudomonas by sodium alginate, and discusses the properties of the immobilized cells, in order to provide reference for the immobilization and application of glutaminase produced by nitroreductive Pseudomonas.