大豆多肽营养干预对预防和治疗尾吊模拟失重大鼠肌肉萎缩的作用

目的:通过尾吊大鼠模拟失重效应,探讨大豆多肽营养干预对肌肉萎缩的预防和治疗作用。方法:选取15只雄性Wistar大鼠为预防实验对象,分为地面-对照组、尾吊-对照组、尾吊-肽组,灌胃40 d。选取20只雄性Wistar大鼠为治疗实验对象,5只作为地面-对照组,15只尾吊40 d后放回地面,分为尾吊后-高剂量肽治疗组、尾吊后-中剂量肽治疗组、尾吊后-低剂量肽治疗组,灌胃60 d。预防和治疗实验结束后,称量大鼠体质量、腓肠肌和比目鱼肌湿重,测定血清中蛋白质合成相关因子(IGF-1)、蛋白质分解相关因子(FoxO3)、氧化应激相关因子(SOD)的浓度及蛋白质分解中关键特异性连接酶(Atrogin-1)的相对表达量。结果:预防实验中,与尾吊-对照组相比,大豆多肽可显著提升大鼠血清中IGF-1浓度(p<0.05),显著降低血清中FoxO3浓度(p<0.05),显著降低比目鱼肌中Atrogin-1蛋白相对表达量(p<0.05)。治疗实验中,与尾吊-对照组相比,不同剂量大豆多肽可显著提高大鼠体质量增长速率(p<0.05),显著提高大鼠血清中IGF-1和SOD浓度(p<0.05),显著降低血清中FoxO3浓度(p<0.05),显著降低腓肠肌和比目鱼肌中Atrogin-1蛋白相对表达量(p<0.05),且治疗60 d后与地面-对照组无显著性差异。结论:大豆多肽营养干预可增强蛋白质合成代谢、减弱蛋白质分解代谢,同时降低体内氧化应激水平,从而一定程度有效预防肌肉萎缩的发生或治疗修复肌肉损伤。

Effect of Soybean Peptides for Nutritional Intervention on Prevention and Treatment for Muscle Atrophy Induced by Stimulating Weightlessness in Tail-Suspension Rats

Purpose: The effect of soybean peptides for nutritional intervention on prevention and treatment for muscle atrophy was investigated by stimulating weightlessness in tail-suspension rats. Methods: 15 male Wistar rats were selected for the prevention experiment and randomly divided into ground control group,tail suspension-model control group and tail suspension-soybean peptide group,and gavaged for 40 d.20 male Wistar rats were selected for the treatment experiment,5 rats were used for ground control group and 15 rats were put back to ground after tail suspension for 40 d,and then divided into tail suspension-high dose group,tail suspension-middle dose group and tail suspension-low dose group,and continuously gavaged for 60 d.After the prevention and treatment experiments,rat total body weight,wet weight of gastrocnemius and soleus muscle were weighed;the concentrations of related factors of protein synthesis( IGF-1),protein proteolysis( FoxO3),oxidative stress( SOD)in rat serum and the relative protein expression of key specific ligase( Atrogin-1) in protein proteolysis were determined.Results: In the prevention experiment,compared with tail suspension-model control group,soybean peptides significantly increased the concentration of IGF-1 in rat serum( p < 0.05),significantly decreased the concentration of FoxO3 in serum( p <0.05) and significantly decreased the relative protein expression of Atrogin-1 in soleus muscle. In the treatment experiment,compared with tail suspension-model control group,soybean peptides with different doses significantly increased the rate of weight gain in rats( p < 0.05),significantly increased the concentrations of IGF-1 and SOD in rat serum( p < 0.05),significantly decreased the concentration of FoxO3 in serum( p < 0.05) and significantly decreased the relative protein expression of Atrogin-1 in both gastrocnemius and soleus muscle( p < 0.05),which were no significant difference with ground control group after recovery for 60 d. Conclusions: Soybean peptides for nutritional intervention can effectively prevent muscle atrophy or improve muscle atrophy by enhancing protein anabolism and reducing protein catabolism to a certain extent,while decrease oxidative stress level in the body.