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多重PCR技术在病原微生物检测中的应用
引用本文:白菊红,康建平,张星灿,华苗苗,刘建,杨健,钟雪婷.多重PCR技术在病原微生物检测中的应用[J].食品工业科技,2019,40(7):322-325,331.
作者姓名:白菊红  康建平  张星灿  华苗苗  刘建  杨健  钟雪婷
作者单位:1. 四川东方主食产业技术研究院, 四川成都 611130;2. 四川省食品发酵工业研究设计院, 四川成都 611130
基金项目:优质川稻(米)绿色化种植与精深加工技术集成硏究与产业化示范(2017NZ0062)。
摘    要:多重PCR技术特异性强、灵敏度高,被广泛应用于生命科学研究的各个领域。本文阐述了多重PCR技术原理,并就其近年来在人类、动物疾病、农作物病害、食源性致病微生物等方面的研究进行了介绍,提出了该技术目前存在的问题,并对其前景进行了展望,旨在为多重PCR技术更好的应用提供参考。

关 键 词:多重PCR    原理    病原微生物检测
收稿时间:2018-08-03

Application of Multiplex PCR Technology on Detection of Pathogenic Microorganism
BAI Jv-hong,KANG Jian-ping,ZHANG Xing-can,HUA Miao-miao,LIU Jian,YANG Jian,ZHONG Xue-ting.Application of Multiplex PCR Technology on Detection of Pathogenic Microorganism[J].Science and Technology of Food Industry,2019,40(7):322-325,331.
Authors:BAI Jv-hong  KANG Jian-ping  ZHANG Xing-can  HUA Miao-miao  LIU Jian  YANG Jian  ZHONG Xue-ting
Affiliation:1. Sichuan Oriental Staple Food Industry Technology Research Institute, Chengdu 611130, China;2. Sichuan Food and Fermentation Industry Research and Design Institute, Chengdu 611130, China
Abstract:Multiplex PCR with high specificity and sensitivity has been widely used in all research areas of life science. The principle and the latest research in the testing of human,animal diseases,crops diseases,food-borne pathogenic microorganism for multiplex PCR in recent years were summarized in this paper. The problems and the prospects were also discussed. The paper was tried to provide references for better application of multiplex PCR.
Keywords:
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