前体和真菌诱导子对大豆悬浮培养细胞中异黄酮积累的影响

以高异黄酮含量的龙野01-491大豆为材料,以下胚轴为外植体诱导愈伤组织,建立大豆细胞悬浮培养体系;并研究了前体(L-phe和NaAc)、真菌诱导子(青霉和黑曲霉诱导子)对大豆悬浮细胞异黄酮积累的影响。结果表明,在细胞培养9 d加入100 μmol/L的L-phe,异黄酮产量达(12.52±0.51) mg/flask,是对照组的3.23倍;在0 d加入80 μmol/L NaAc,产量达(5.55±0.36) mg/flask,是对照的1.43倍;在9 d加入0.5 mL青霉诱导子,异黄酮产量达(10.20±0.17) mg/flask,是对照的2.56倍;在9 d加入0.5 mL黑曲霉诱导子,产量达(7.25±0.30) mg/flask,是对照的1.82倍。在大豆细胞悬浮培养过程中,通过添加前体(L-phe和NaAc)和真菌诱导子(青霉和黑曲霉诱导子)可以促进大豆异黄酮的积累。

Effects of Precursors and Fungal Elicitors on the Accumulation of Isoflavones in Cell Suspension Culture of Soybean

To establish the cell suspension culture of soybean,LongYe 01-491 was used as materials,and its hypocotyl was induced to the callus. Different concentrations of the precursors(sodium acetate and L-phenylalanine),and fungi elicitors(Penicillium and Aspergillus niger)were added into the cell suspension culture to investigate their effects on the accumulation of isoflavones in cell suspension culture. The results showed that when 100 μmol/L L-phe was added to the suspended cells on the 9 th day,the yield of soybean isoflavones was up to(12.52±0.51) mg/flask which was 3.23 times of the control group;When 80 μmol/L NaAc was added to the suspended cells on the 0 th day,the yield of soybean isoflavones was up to(5.55±0.36) mg/flask which was 1.43 times of the control group;When 0.5 mL elicitor of Penicillium was added to the suspended cells on the 9 th day,the yield of soybean isoflavones was up to(10.20±0.17) mg/flask,which was 2.56 times of the control group;When 0.5 mL elicitor of Aspergillus niger was added to the suspended cells at the 9 th day,the yield of soybean isoflavones was up to(7.25±0.30) mg/flask,which was 1.82 times of the control group. In conclusion,the addition of precursors(sodium acetate and L-phenylalanine)and the fungal elicitors(Penicillium and Aspergillus niger)can increase the accumulation of soybean isoflavones in suspended culture of soybean.