固相萃取-高效液相色谱法同时测定食品中16种禁限用色素

目的建立一种高效、准确、同时测定食品中16种禁限用色素(柠檬黄、新红、苋菜红、胭脂红、日落黄、酸性品红6B、诱惑红、酸性红13、丽春红2R、丽春红SX、酸性橙I、赤藓红、酸性黄11、酸性蓝113、罗丹明B、分散红1)的方法。方法待测样品先后经0.5%氨水和乙醇+氨水+水提取、经OasisHLB固相萃取柱净化后,以Eclipse XDB C18(150 mm×4.6 mm, 5?m)为分离柱,甲醇和10 mmol/L乙酸铵为流动相进行梯度洗脱,流速为0.8 mL/min,检测波长为470、520、550和490 nm的条件下用高效液相色谱法进行测定,外标法定量。结果 16种禁限用色素在此次方法下有较好的线性关系(r≥0.9997),检出限(S/N=3)为33.0～144.0μg/L。结论本研究建立的固相萃取-高效液相色谱法可以同时测定食品中16种禁限用色素,且方法高效、准确。

Simultaneous determination of 16 prohibited pigments in food by solid phase extraction and high performance liquid chromatography

Objective To establish an efficient, accurate and simultaneous determination of 16 prohibited and restricted pigments in food (lemon yellow, new red, amaranth, carmine, sunset yellow, acid fuchsin 6B, temptation red, acid red 13, Ponceau 2R, Ponceau SX, acid orange I, erythrin, acid yellow 11, acid blue 113, rhodamine B, disperse red 1). Methods The samples were extracted by 0.5% ammonia water and ethanol+ammonia water and purified by Oasis HLB solid phase extraction column, and then determined by high performance liquid chromatography and quantified by external standard method. The separation column was Eclipse XDB C18 (150 mm×4.6 mm, 5 ?m), methanol and 10 mmol/L ammonium acetate as mobile phase for gradient elution. The flow rate was 0.8 mL/min, and the detection wavelength was 470, 520, 550 and 490 nm. Results Under this method, there was a good linear relationship between the 16 prohibited and restricted pigments (r<0.9997), and the detection limit (S/N=3) was 33.0?144.0 g/L. Conclusion The established solid phase extraction-high performance liquid chromatography method can simultaneously determine 16 prohibited and restricted pigments in food with high efficiency and accuracy.