乳源外泌体的提取及鉴定方法研究进展

乳外泌体是乳中纳米级的胞外囊泡,不仅能够作为药物载体,而且具有促进肠道发育、免疫调节等功能,在婴幼儿配方食品以及功能性食品开发方面具有极大的潜力。外泌体含量低是限制其广泛应用的瓶颈之一。目前,外泌体提取及鉴定的方法尚没有统一的标准,选择合适的乳外泌体提取、鉴定方法对后续的深入研究十分关键。据此,本文综述了目前乳外泌体的提取及鉴定方法,探讨了尚未应用于乳外泌体提取鉴定但理论可行的新方法,期望为乳外泌体的研究与开发提供理论参考。

Advances in extraction and identification methods of milk-derived exosomes

Milk-derived exosomes are nano-scale extracellular vesicles in milk. They can not only be used as a drug carrier but also promote intestinal development and immune regulation. Milk-derived exosomes have great potential for use in infant formula and functional foods in the future. Low yield is one of the bottle neck for the development of milk-derived exosomes. At present, there is no uniform standard for methods for extraction and identification of exosomes, so it is very important to select appropriate methods for the extraction and identification of milk exosomes for subsequent studies. Based on the above research progress, this paper reviewed the current methods of extraction and identification of exosomes from milk, and discussed new methods those had not been applied to the extraction and identification of exosomes from milk. It is expected to provide a theoretical reference for the research and development of milk-derived exosomes.