固相萃取-高效液相色谱法测定食品基质中5种不同合成红色着色剂

目的建立一种固相萃取-高效液相色谱法(solidphaseextraction-highperformanceliquid chromatography,SPE-HPLC)测定食品中5种人工合成红色着色剂(新红、苋菜红、胭脂红、诱惑红、赤藓红)的方法。方法比较ProElut PWA-2, Cleanert PWAX和Sep-Pak Plus QMA 3种不同SPE小柱的净化效果,比较Eclipse XDB C_(18)、Venusil XBP C_(18)及Agilent TC-C_(18) 3种色谱柱对5种着色剂的分离情况,按照GB 5009.35-2016《食品中合成着色剂的测定》对食品中5种着色剂进行测定。结果 Pro Elut PWA-2 SPE固相萃取小柱进行样品净化效果最优, Agilent TC-C_(18)色谱柱在10 min内有较好的分离度。加标浓度为0.5～50 mg/kg时,该方法回收率为90.3%～103.9%,相对标准偏差(relative standard deviation, RSD)值为1.18%～7.11%。结论该方法快速、准确,适合用于不同食品基质中5种合成着色剂定性、定量测定。

Determination of 5 kinds of different synthetic red colorants in food substrates by solid phase extraction-high performance liquid chromatography

Objective To establish a method for determination of 5 kinds of synthetic red colorants (new red, amaranth, carmine, temptation red and erythromycin) in food substrates by solid phase extraction-high performance liquid chromatography. Methods The purification effects of three different SPE cartridges (ProElut PWA-2, Cleanert PWAX and Sep-Pak Plus QMA) were compared, and the separation of 5 colorants by Eclipse XDB C18, Venusil XBP C18 and Agilent TC-C18 columns were compared. Five kinds of colorants in food were detected according to GB 5009.35-2016 Determination of synthetic colorants in food. Results The ProElut PWA-2 SPE solid phase extraction cartridge provided the best sample purification. The Agilent TC-C18 column had good resolution in 10 min. When the concentrations were 0.5-50 mg/kg, the recovery rates of the method were 90.3%-103.9%, and the relative standard deviations (RSDs) were 1.18%-7.11%. Conclusion This method is rapid and accurate, which is suitable for qualitative and quantitative determination of 5 kinds of synthetic colorants in different food matrices.