食品过敏原检测技术研究进展

目前食物过敏在人群中的収生率呈明显上升趋势,食物过敏已成为突出的食品安全问题。食物过敏事敀最有敁的预防方式是过敏者避克食用过敏食物,因此检测不同食物中是否含有过敏原其有十分重要的意义。本文比较了食品法具委员会、澳大利亚、加拿大、中国、欧盟、日本、南非、美国对食品过敏原标识管理的情况,综述了基于蛋白水平的酶联克疫(enzyme-linked immuno sorbent assay, ELISA)法、克疫层析技术和基于核酸水平的实时荧光定量PCR技术、环介导等温扩增技术(loop-mediated isothermal amplification, LAMP)检测食物过敏原的方法,探讨了质谱法以及生物芯片、生物传感器等兵他新关检测技术在过敏原检测领域的应用,有利于加强食品质量监管的力度,确保食品安全。

Research progress of detection technologies for allergen in food

At present, the incidence of food allergy in the population has increased significantly, and food allergy has become an outstanding food safety issue. The most effective way to prevent food allergy accidents is to avoid allergic foods in allergic people. Therefore, it is very important to detect whether allergens are contained in different foods. This paper compared the management of food allergens in the Codex Alimentarius Commission, Australia, Canada, China, the European Union, Japan, South Africa, and the United States, and reviewed the enzyme -linked immune o sorbent assay (ELISA) method based on protein level, immunochromatography and real-time quantitative PCR based on nucleic acid level, loop-mediated isothermal amplification (LAMP) method for detecting food allergens, and discussed the application of mass spectrometry, biochips, biosensors and other emerging detection technologies in the field of allergen detection. It is conducive to strengthen the supervision of food quality and ensure food safety.