| 多头绒泡菌细胞核肌动蛋白相对含量的动态变化 |
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| 引用本文： | 宋朝霞,邢苗. 多头绒泡菌细胞核肌动蛋白相对含量的动态变化[J]. 深圳大学学报(理工版), 2001, 18(2): 34-38 |
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| 作者姓名： | 宋朝霞 邢苗 |
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| 作者单位： | 1. 白求恩医科大学基础医学院遗传教研室,
2. 深圳大学生物工程系, |
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| 基金项目： | 国家自然科学基金资助项目（39870389） |
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| 摘 要： | 将自然同步化的多头绒泡菌生质团中的S期、G2早期、G2中期和G2晚期细胞核提取出来，经SDS－PAGE发现一条分子量为43KD的多肽，经Westen blot证明为肌动蛋白，凝胶染色后，发现细胞周期不同期相的肌动蛋白带着深浅不同。G2早期染色最深，G2晚期染色最浅，电泳凝胶扫描表明肌动蛋白相对含量依细胞周期相不同而具有动态变化；G2早期相对含量最高，达6．9％；G2晚期相对含量最低。为2．3％。
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| 关 键 词： | 细胞周期 细胞核肌动蛋白 相对含量 多头绒泡菌 |
| 文章编号： | 1000-2618(2001)02-0034-05 |
| 修稿时间： | 2001-04-18 |
Dynamic Changes in the Relative Content of Nuclear Actin during Cell Cycle of Physarum Polycephalum |
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| SONG Zhao-xia XING Miao. Dynamic Changes in the Relative Content of Nuclear Actin during Cell Cycle of Physarum Polycephalum[J]. Journal of Shenzhen University(Science &engineering), 2001, 18(2): 34-38 |
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| Authors: | SONG Zhao-xia XING Miao |
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| Affiliation: | SONG Zhao-xia XING Miao Department of Medical GeneticsDepartment of Biotechnology School of Basic Medical Sciences Shenzhen University Norman Bethune University of Medical Sciences Shenzhen,518060 Changchun 130023 P.R.China |
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| Abstract: | Nuclei at S, early G2, middle G2, late G2 and M phases of the cell cycle were isolated from the naturally synchronized plasmodia of Physarum polycephalum. A 43kD polypeptide was revealed in the nuclear proteins of all the phases by SDS PAGE and was then proved to be actin by Western blot analysis. The actin bands in different phases showed a difference in their darkness after staining: the darkest was found in early G2 phase while the lightest turned to be in the late G2 phase. Gelscan of the SDS PAGE gels indicated a phase dependent dynamic change in the relative content of actin: it reached the maximum 6 9% of the whole nuclear proteins in the early G2 phase and then dropped to the minimum 2 3% in the late G2 phase. |
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| Keywords: | cell cycle nuclear actin relative content Physarum polycephalum |
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