Salmon fibrinogen and chitosan scaffold for tissue engineering: in vitro and in vivo evaluation |
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| Authors: | Ivo Laidmäe Kaspars Ērglis Andrejs Cēbers Paul A. Janmey Raivo Uibo |
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| Affiliation: | 1.Department of Immunology, Institute of Biomedicine and Translational Medicine,University of Tartu,Tartu,Estonia;2.Institute of Pharmacy,University of Tartu,Tartu,Estonia;3.Faculty of Physics, Mathematics and Optometry,University of Latvia,Riga,Latvia;4.Institute for Medicine and Engineering and Center for Engineering Mechanobiology,University of Pennsylvania,Philadelphia,USA |
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| Abstract: | 3D fibrous scaffolds have received much recent attention in regenerative medicine. Use of fibrous scaffolds has shown promising results in tissue engineering and wound healing. Here we report the development and properties of a novel fibrous scaffold that is useful for promoting wound healing. A scaffold made of salmon fibrinogen and chitosan is produced by electrospinning, resulting in a biocompatible material mimicking the structure of the native extracellular matrix (ECM) with suitable biochemical and mechanical properties. The scaffold is produced without the need for enzymes, in particular thrombin, but is fully compatible with their addition if needed. Human dermal fibroblasts cultured on this scaffold showed progressive proliferation for 14 days. Split-thickness experimental skin wounds treated and untreated were compared in a 10-day follow-up period. Wound healing was more effective using the fibrinogen-chitosan scaffold than in untreated wounds. This scaffold could be applicable in various medical purposes including surgery, tissue regeneration, burns, traumatic injuries, and 3D cell culture platforms. | |
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