TOTAL β-GLUCAN CONTENT OF SOME BARLEY CULTIVARS |
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Authors: | A. A. Gill A. G. Morgan D. B. Smith |
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Abstract: | The use of cellulase preparations from Trichoderma reesii for measuring the total β-glucan content of barley was examined. The activities of amyloglucosidase and β-glucanase in the cellulase varied considerably between batches, and different heat treatments were necessary to ensure that amyloglucosidase was reduced to an insignificant level while adequate β-glucanase activity was retained. After suitable treatment the cellulase was used to study variation of total β-glucan concentration in some barley cultivars. Significant varietal variation was found in the fifteen genotypes examined. These had β-glucan concentrations in the range 2.7% to 5.2% dry weight. |
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Keywords: | total β -glucan barley β -glucanase amyloglucosidase |
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