Comparative study on ethanol production from pretreated sugarcane bagasse using immobilized Saccharomyces cerevisiae on various matrices |
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| Affiliation: | 1. Department of Environmental Science and Engineering, Guru Jambheshwar University of Science and Technology, Hisar-125001, Haryana, India;2. Department of Bio and Nano Technology, Guru Jambheshwar University of Science and Technology, Hisar-125001, Haryana, India;1. Graduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodai, Nada-ku, Kobe 657-8501, Japan;2. Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada-ku, Kobe 657-8501, Japan;3. Division of Material Engineering, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan;4. Institute of Nature and Environmental Technology, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan;5. Department of Chemical Engineering, Osaka Prefecture University, 1-1 Gakuencho, Naka-ku, Sakai, Osaka 599-8531, Japan;1. Research Group on Biomass Energy, Department of Nuclear Energy, Federal University of Pernambuco, 50740-540, Recife, PE, Brazil;2. Technical School Professor Agamenon Magalhães (ETEPAM), 52050-385, Recife, PE, Brazil;3. Interdepartmental Research Group in Metabolic Engineering, Department of Genetics, Federal University of Pernambuco, 50670-901, Recife, PE, Brazil;4. White Biotechnology Group, Bioprocess Laboratory, Center for Strategic Technology of the Northeast (CETENE), 50.740-540, Recife, PE, Brazil;5. Antibiotics Department (DANTI), Federal University of Pernambuco (UFPE), 50740-525, Recife, PE, Brazil;1. Research Unit for Development of Chemical Engineering Processes – Indonesian Institute of Sciences. Jl. Yogya-Wonosari KM 31.5 Gading, Playen, Gunungkidul, Yogyakarta 55861, Indonesia;2. Education of Chemistry Department – Sebelas Maret University, Solo, Jl.Ir Sutami 36 A Kentingan, Surakarta 57126, Indonesia;1. Department of Biotechnology, Engineering School of Lorena, University of São Paulo, CEP 12602-810, Brazil;2. Department of Civil and Environmental Engineering, KAIST, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea |
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| Abstract: | Microwave alkali pretreated sugarcane bagasse was used as a substrate for production of cellulolytic enzymes, needed for biomass hydrolysis. The pretreated sugarcane bagasse was enzymatic hydrolyzed by crude unprocessed enzymes cellulase (Filter paper activity 9.4 FPU/g), endoglucanase (carboxymethylcellulase, 148 IU/g), β-glucosidase (116 IU/g) and xylanase (201 IU/g) produced by Aspergillus flavus using pretreated sugarcane bagasse as substrate under solid state fermentation. Concentrated enzymatic hydrolyzate was used for ethanol production using Saccharomyces cerevisiae immobilized on various matrices. The yield of ethanol was 0.44 gp/gs in case of yeast immobilized sugarcane bagasse, 0.38 gp/gs using Ca-alginate and 0.33 gp/gs using agar-agar as immobilization matrices. The immobilized yeast studied up to 10 cycles in case of immobilized sugarcane bagasse and up to 4 cycles in case of agar-agar and calcium alginate for ethanol production under repeated batch fermentation study. |
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