Screening and optimization of trace elements supplement in sweet sorghum juice for ethanol production |
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| Affiliation: | 1. Department of Molecular Biosciences, University of Kansas, 1200 Sunnyside Ave, Lawrence, KS 66045, United States;2. Department of Chemistry, University of Kansas, 1251 Wescoe Hall Dr, Lawrence, KS 66045, United States;3. High Throughput Screening Facility, University of Kansas, 2034 Becker Dr, Lawrence, KS 66047, United States;4. Department of Medicinal Chemistry, University of Kansas, 1251 Wescoe Hall Dr, Lawrence, KS 66045, United States;1. EA 4267 (FDE/UFC), Laboratoire de Pharmacognosie, Faculté de Pharmacie, Université de Bourgogne, 7, Bd. Jeanne d’Arc, BP 87900, 21079 Dijon Cedex, France;2. Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812–8582, Japan;3. Cohiro, UFR de Médecine de Dijon, 7, Bd. Jeanne d’Arc, BP 87900, 21079 Dijon Cedex, France;1. Bioenergy Crop Research Institute, National Institute of Crop Science, Rural Development Administration, Muan-ro 199, Muan 534-833, Korea;2. School of Life Sciences and Biotechnology for BK21 PLUS, Korea University, Seoul 136-713, Korea;1. Empa, Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Applied Wood Materials, Lerchenfeldstrasse 5, CH-9014 St. Gallen, Switzerland;2. Empa, Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Bioactive Surfaces, Lerchenfeldstrasse 5, CH-9014 St. Gallen, Switzerland;3. Institute for Building Materials, ETH Zurich, Wolfgang-Pauli Strasse 10, CH-8093 Zurich, Switzerland;4. Process Technology Development, R+D, FISBA OPTIK AG, Rorschacherstrasse 268, 9016 St. Gallen, Switzerland |
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| Abstract: | Eight trace elements were screened for increasing efficiency of ethanol yield from sweet sorghum juice using the Plackett-Burman design method. MnCl2·4H2O, CoCl2·6H2O and biotin was screened as the significant variables which have positive effects on ethanol production from sweet sorghum juice. The values of MnCl2·4H2O, CoCl2·6H2O and biotin, optimized by Box-Behnken design method, were 7.70 mg L?1, 15.74 mg L?1 and 11.97 mg L?1, respectively. The experimental efficiency of ethanol yield under optimal conditions was 89.30 ± 0.10%, which enhances the efficiency of ethanol yield by 5.63% by the addition of MnCl2·4H2O, CoCl2·6H2O and biotin. The results from this study have identified optimal conditions as a foundation for pilot scale ethanol production. |
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