Fermentative hydrogen production by a new mesophilic bacterium Clostridium sp. 6A-5 isolated from the sludge of a sugar mill |
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| Affiliation: | 1. Tianjin Key Laboratory of Marine Resources and Chemistry, College of Marine Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, PR China;2. Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai, Shinan District, Qingdao 266071, Shandong, PR China;3. Guangxi Zhuang Autonomous Region Forestry Research Institute, Nanning 530001, Guangxi, PR China;4. Environmental Monitoring Center, Environmental Protection Department, Nanning 530022, Guangxi, PR China;1. Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Cx. P. 19046 Centro Politécnico, Curitiba 81531-980, Paraná, Brazil;2. Núcleo de Pesquisa e Desenvolvimento de Energia Autossustentável, Universidade Federal do Paraná, Curitiba 81531-980, Paraná, Brazil;3. Departamento de Engenharia Química, Universidade Federal de Santa Catarina, Florianópolis 88040-970, Santa Catarina, Brazil;4. Departmento de Engenharia Química, Universidade Federal do Paraná, Cx. P. 19011 Centro Politécnico, Curitiba 81531-980, Paraná, Brazil;5. Departamento de Química, Universidade Federal do Paraná, Cx. P. 19081 Centro Politécnico, Curitiba 81531-980, Paraná, Brazil;1. The Biosensor and Bioelectronics Technology Centre, King Mongkut''s University of Technology North Bangkok, 10800, Thailand;2. Department of Agro-Industrial, Food and Environmental Technology, Faculty of Applied Science, King Mongkut''s University of Technology North Bangkok, 10800, Thailand;3. The Research and Technology Center for Renewable Products and Energy, King Mongkut''s University of Technology North Bangkok, Bangkok, 10800, Thailand;1. Department of Environmental Science and Engineering, Beijing University of Chemical Technology, 15 Beisanhuan East Road, Chaoyang District, Beijing 100029, China;2. State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China;3. China Power Conservation & Environment Protection CO., LTD, Beijing 100020, China;1. Department of Bioproducts and Biosystems Engineering, University of Minnesota, 1390 Eckles Ave., St. Paul, MN 55108, USA;2. State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China;3. Shanghai Institute of Space Propulsion, Shanghai 201112, China;1. State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China;2. Department of Civil and Environmental Engineering, Tohoku University, Sendai 9808579, Japan |
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| Abstract: | The fermentative hydrogen production capability of the newly isolated Clostridium sp. 6A-5 bacterium was studied in a batch cultivation experiment. Various culture conditions (temperature, initial pH, and glucose concentration) were evaluated for their effects on cell growth and hydrogen production (including the yield and rate) of Clostridium sp. 6A-5. Optimal cell growth was observed at 40 °C, initial pH 7.5–8, and glucose concentration 16–26 g/L. The optimal hydrogen yield was obtained at 43 °C, initial pH 8, and glucose concentration 10–16 g/L. Hydrogen began to evolve when cell growth entered the mid-exponential phase and reached the maximum production rate at the late exponential and stationary phases. The maximum hydrogen yield, and rate were 2727 mL/L, and 269.3 mL H2/L h, respectively. These results indicate that Clostridium sp. 6A-5 is a good candidate for mesophilic fermentative hydrogen production. |
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| Keywords: | Biohydrogen production Isolation Mesophilic Culture conditions |
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