Optimization of biohydrogen production by Clostridium butyricum EB6 from palm oil mill effluent using response surface methodology |
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| Authors: | Mei-Ling Chong Nor' Aini Abdul Rahman Raha Abdul Rahim Suraini Abdul Aziz Yoshihito Shirai Mohd Ali Hassan |
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| Affiliation: | aDepartment of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia;bDepartment of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia;cGraduate School of Life Sciences and System Engineering, Kyushu Institute of Technology, 808-0196 Hibikino 2-4, Wakamatsu-ku, Kitakyushu-shi, Fukuoka, Japan |
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| Abstract: | Clostridium butyricum EB6 successfully produced hydrogen gas from palm oil mill effluent (POME). In this study, central composite design and response surface methodology were applied to determine the optimum conditions for hydrogen production (Pc) and maximum hydrogen production rate (Rmax) from POME. Experimental results showed that the pH, temperature and chemical oxygen demand (COD) of POME affected both the hydrogen production and production rate, both individually and interactively. The optimum conditions for hydrogen production (Pc) were pH 5.69, 36 °C, and 92 g COD/l; with an estimated Pc value of 306 ml H2/g carbohydrate. The optimum conditions for maximum hydrogen production rate (Rmax) were pH 6.52, 41 °C and 60 g COD/l; with an estimated Rmax value of 914 ml H2/h. An overlay study was performed to obtain an overall model optimization. The optimized conditions for the overall model were pH 6.05, 36 °C and 94 g COD/l. The hydrogen content in the biogas produced ranged from 60% to 75%. |
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| Keywords: | Biohydrogen Clostridium butyricum EB6 Response surface methodology POME |
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