超高效液相色谱-串联质谱法同时测定水产制品中22种全氟烷基物质

目的 建立超高效液相色谱-串联质谱法同时检测水产制品中22种全氟烷基物质(perfluorinated alkyl substances,PFASs)定性定量检测方法.方法 选取基质成分复杂的水产制品作为检测目标.样品经乙腈(含1％甲酸)提取液涡旋振荡提取,分散固相萃取结合通过式固相萃取柱进行净化,C18色谱柱分离,甲...

Simultaneous determination of 22 kinds of perfluorinated alkyl substances in aquatic products by ultra performance liquid chromatography-tandem mass spectrometry

Objective To establish a qualitative and quantitative method for the simultaneous determination of 22 kinds of perfluorinated alkyl substances(PFASs) in aquatic products by ultra performance liquid chromatography-tandem mass spectrometry. Methods Aquatic products with complex matrix components were selected as the detection target. The samples were extracted by vortex oscillation from 1% formic acid-acetonitrile extract, the disper solid phase extraction was combined with purification through a type solid phase extraction column,the samples were separated by a C₁₈ column, and then eluted by gradient elution with methanol and 5 mmol/L ammonium acetate solution, the samples were collected by multiple reaction monitoring negative ion mode, and quantified by isotope internal standard method. Results The linear relationships of the 22 kinds of PFASs were good within the corresponding mass concentrations, with the correlation coefficients greater than 0.994 and the limits of quantification were 0.10-0.25 μg/kg. The recoveries of 2 kinds of samples at spiked levels(the limit of detection,1.0 μg/kg, 10.0 μg/kg) were 71.8%-98.2%, the relative standard deviations were 3.9%-10.9%. Conclusion This method has the advantages of strong anti-interference, simple pretreatment operation, fast analysis speed and high sensitivity, and is suitable for the rapid and accurate detection of 22 kinds of PFASs in aquatic products.