恩诺沙星在余干乌鸡不同组织中的残留消除规律研究

目的 研究恩诺沙星可溶性粉在余干乌鸡各组织内的残留消除规律。方法 88只110d龄健康余干乌鸡,随机分为11组,以0.75 g/L饮水连续给药5 d。停药后第0.16、1、3、5、7、9、20、30、40 d采集肌肉、肝脏、肾脏、皮脂样本,经液相色谱-串联质谱法测定组织中恩诺沙星和环丙沙星残留量,计算药物代谢半衰期,并利用WT1.4软件计算恩诺沙星在余干乌鸡体内的休药期。结果 恩诺沙星在余干乌鸡体内的半衰期和休药期均长于普通肉鸡。恩诺沙星可溶性粉在余干乌鸡肌肉、肝脏、肾脏及皮脂中的休药期分别是110、64、67和208 d。结论 为保证乌鸡产品质量安全和消费者健康,推荐恩诺沙星可溶性粉在余干乌鸡体内的休药期应大于210 d。

Study on residue elimination rule of enrofloxacin in different tissues of Yugan black-bone fowl

Objective In order to study the residual rule of enrofloxacin soluble powder in various tissues of Yugan Black-bone fowl. Methods Eighty-eight 110-day-old healthy Yugan Black-bone Fowls were randomly divided into 11 groups and given 0.75 g/L drinking water continuously for 5 days. Samples of muscle, liver, kidney and sebum were collected at 0.16, 1, 3, 5, 7, 9, 20, 30 and 40 days after drug withdrawal.The residues of enrofloxacin and ciprofloxacin in tissues were determined by liquid chromatography-tandem mass spectrometry. The half-life of drug metabolism was calculated, and the drug withdrawal period of enrofloxacin in Yugan Black-bone fowl was calculated by WT1.4 software. Results Enrofloxacin has high affinity with melanin, and its half-life and drug withdrawal period in Yugan Black-bone fowl are longer than those in common broilers. The withdrawal period of enrofloxacin soluble powder in muscle, liver, kidney and sebum of Yugan Black-bone fowl was 110 d, 64 d, 67 d and 208 d respectively. Conclusion To ensure the quality and safety of black-bone chicken products and the health of consumers, it is recommended that the drug withdrawal period of enrofloxacin soluble powder in Yugan Black-bone fowl should be greater than 210 days.